FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004925421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4367401
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT51345211.756465687487822Illumina Single End PCR Primer 1 (100% over 50bp)
GCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCTC75670.17326093940080153No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC75400.17264272275433373No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG72110.16510963843255977Illumina Single End PCR Primer 1 (100% over 50bp)
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG66240.15166915060009376No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC53840.12327697868824043No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG52620.12048355532271939No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT48210.11038601676374576No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA46960.10752390265972829No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)