FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17945192
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1905191.0616715608281038Illumina Single End PCR Primer 1 (100% over 50bp)
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1414500.7882334165051007No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT909350.5067374035340496No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG604430.3368200239930562No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT549200.30604297797426744No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC539910.3008661038566765No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC526500.29339335015195156No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT510120.28426555703611306No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA495750.27625784109749285No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG447980.24963789743793213No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT422060.2351939171227591No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT402800.22446123730523473No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT392650.21880512618644593No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG360080.20065541789689406No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC358930.20001457772087367No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC349380.1946928179982694No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT344300.1918619761772401No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT313180.17452028376180095No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA309510.17247516772180538No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT286520.15966393672466697No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT282940.1576689733940991No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC272460.15182896900740878No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC267640.1491430127913928No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG267440.14903156232599796No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT266510.1485133176619119No Hit
CCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAA249030.1387725469864017No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT242640.13521170461703616No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA241150.1343813986498445No Hit
CTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCT234970.1309375792691435No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG234400.13061994544276817No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA220560.12290757323744432No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA218300.12164818297848248No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG217410.12115222840747537No Hit
TAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTT216450.12061726617358008No Hit
GCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCTC204220.1138020702146848No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA201880.11249809976956501No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT191850.10690885893001312No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC191820.10689214136020389No Hit
CATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAA188010.10476900999443194No Hit
CCCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTAC185120.1031585507694763No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC182560.10173198481242217No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)