FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21096359
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC702070.33279202349561837No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC413900.19619499269992513No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA336930.1597100238955926No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG307340.14568390687701135No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG281410.13339268638725763No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA262230.12430107015148917No Hit
CAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCCAAGGCAA259430.12297382690539159No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA250860.11891151454144291No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT234790.11129408633973284No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA233680.11076792919574416No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT223760.10606569598099842No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)