FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17945192
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1824081.016472824587221TruSeq Adapter, Index 13 (97% over 39bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG531570.2962186194497111No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA299870.16710325528977343No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA275860.15372362691912128No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA254090.1415922437608915No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT214620.11959749441521718No Hit
CTAAGAAGAATTTTATGGAGAAAGGGACGCGGGCGGGGGATATAGGGTCG193070.10758870676892172No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG187960.10474114737808324No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG186360.1038495436549244No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA184750.10295236740849582No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT182080.10146450369547452No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)