FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16682065
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT764750.4584264597937965No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA631950.3788200081944292No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT548890.3290300091745237No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC478480.28682300422639523No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG408550.24490373344067418No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT396670.23778231292109223No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG383070.22962984498621722No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA353570.21194618292159875No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG348640.20899091329520655No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT324040.19424453747182976No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC306330.18362834577134185No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC296770.1778976403700621No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC294410.17648294740489262No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT289640.17362358916596957No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA276060.1654831101545282No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA250200.14998143215483214No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT228940.1372372065448732No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT224880.13480345508784436No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT224750.13472552708552568No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC220470.1321598974707268No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG213180.12778993487916515No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC212000.1270825883965804No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA211480.12677087638730578No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT210030.1259016794383669No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC203840.12219110763565541No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG202190.12120202145238014No Hit
GCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCTC198050.11872031430161674No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG173760.10415976679146136No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG173610.10406984986570907No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG168140.10079087930660861No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)