FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15756013
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA644980.4093548285343506No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT481440.30555953463607827No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC474130.3009200360522678No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA470610.29868596833475575No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG420610.2669520518928234No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG366620.23268576891882484No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT352530.22374315126548827No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC305310.1937736405777274No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG269870.17128064060368572No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT268890.17065865584142384No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC267980.17008109856218068No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT251320.15950735760372883No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG248340.15761601618378965No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT237870.150970934080849No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC227980.14469396540863477No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA224080.14221871992616408No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT207820.13189885029924767No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG207480.1316830596674425No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC203010.12884604753753376No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG200170.127043561083632No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA199530.12663736695317526No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA186750.11852617791061737No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC180630.11464194653812484No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA170010.10790166268585841No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA168430.10689887092629334No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG166000.10535660258721544No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT162880.10337640620123885No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC162550.1031669623527221No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC160000.10154853261418355No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)