FastQCFastQC Report
Sun 5 May 2024
EGAF00004925698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21375170
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT1002040.4687869149110861No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT841020.39345651987797053No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT765920.35832229638407553No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT576560.26973352726551414No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT517080.24190684799232004No Hit
CGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCT495440.23178295190166906No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT388940.18195878676052635No Hit
CAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTT371330.17372025579211767No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT350360.1639098075009462No Hit
CTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCTCTTTA302430.14148659402474928No Hit
GTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCC294810.13792171009634077No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC255150.11936747169730112No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT251560.1176879528911349No Hit
TGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCC241340.11290670436773134No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC234660.10978158302366718No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT226950.10617459416697037Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)