FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21837718
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC2105360.9640934093937837No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT2069850.9478325528335881No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1313350.6014135726086398No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC1042530.47739878315124323No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA813420.37248397474498024No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG738500.33817636073512813No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT697570.31943355986188665No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT679320.31107645954581886No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT612210.28034522654793875No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC610400.27951638536590684No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT594000.2720064431640705No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT593700.2718690661725735No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA577370.2643911786020865No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC550730.2521921017571525No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG544690.24942624499501276No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT542260.248313491363887No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA528350.24194377819147586No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT523010.23949846774282915No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC510290.23367368330335617No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA497180.227670308774937No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG490070.22441447407645798No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC476360.21813634556504483No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA467020.21385934189643807No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT456150.2088817155711966No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT436300.19979193796714473No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC419400.19205303411281344No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA401970.18407143090683742No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG385840.17668512799734842No Hit
TTTTAATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTT360270.1649760290887537No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC359320.16454100194901317No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG352860.16158281739877767No Hit
CTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCT350450.16047922223375172No Hit
CCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAA342070.15664182493793538No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA331500.15180157560419086No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG305910.14008331822949632No Hit
CAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAA303270.1388744007043227No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA303090.1387919745094245No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT301560.13809135185278973No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA299240.1370289697852129No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT298130.13652067491667397No Hit
TGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCT294820.13500494877715702No Hit
GCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCTC288160.1319551795659235No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC265800.12171601446634671No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA250170.11455867320935274No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG244910.11214999662510525No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC234600.10742880735065816No Hit
GTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCAC234200.10724563802866215No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG234070.10718610799901344No Hit
ATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTC233960.10713573643546453No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC231360.1059451358424905No Hit
ACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCAT230690.10563832722814719No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA229140.10492854610541266No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)