FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12551825
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA468580.373316230906661No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA430710.3431453195053309No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC428520.34140055330599334No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG420320.33486763876966097No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT413530.32945806685481993No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT410260.32685286800923374No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT362260.28861141706484916No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC334450.2664552764239463No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA320780.25556442987374345No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT287070.22870777755426003No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC274550.21873313243293305No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT270820.21576145301579652No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC264380.21063072501409158No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG262770.20934804301366533No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT261540.2083681058332155No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG260910.20786618678957045No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA228520.1820611743710576No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT222120.17696231424513964No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT210430.16764892754639266No Hit
GCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCTC204370.1628209443646641No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG194780.15518062114473394No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC192940.15371469885853253No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA188680.1503207700872184No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA186280.14840869753999916No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG185910.14811391968896953No Hit
TGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGC184470.146966676160638No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT173860.13851372210813964No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG171880.1369362622566838No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT163190.13001296624196082No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA159770.12728826286217343No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT156630.12478663461289494No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC152750.1216954506615572No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA150720.12007815596536758No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG149170.11884327577862185No Hit
TAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATAT139810.11138619284446684No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC138680.11048592535348445No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG133810.10660601147641877No Hit
CTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACTATTCCTC131700.1049249810286552No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG130230.10375383659348343No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC128750.1025747251893649No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC126940.10113270381000372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)