FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16161958
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA3726972.306013912423235No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG2263591.4005666887638242No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG2155791.3338668495487986No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT1771041.0958078222947987No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG1603800.9923302609745676No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG1461040.903999379283129No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG1339370.8287176590856133No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC1077160.6664786531433877No Hit
TGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTC1001260.6195165214511756No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT815150.5043633945837503No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC572580.3542763816116834No Hit
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG529430.32757788381828484No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT507600.3140708570087857No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG506630.31347068220323304No Hit
GCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGT470750.29127040176691466No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC430410.26631055469887993No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT407170.2519311088421341No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC397630.2460283586926782No Hit
GTGTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA396460.24530443650453737No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT389440.24096090337569248No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC386520.23915419158990514No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG386260.2389933199925405No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA349410.21619286475066946No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC332890.20597133094888628No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT332700.2058537709354275No Hit
CGCGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGG330270.2043502402369812No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC317890.19669027725477323No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT307160.19005123017891767No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG301590.18660486557383701No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG301440.1865120550368959No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA295580.1828862567270624No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC295130.182607825116239No Hit
GACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCC288580.17855509833647631No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG283930.17567797169130125No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG276300.1709570090455624No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA275720.17059814163605672No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG269130.1665206653797764No Hit
CCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCG251340.1555133356985583No Hit
CGGTGTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG248810.15394793130881793No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT244980.15157816893225437Illumina Single End PCR Primer 1 (100% over 50bp)
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA243780.1508356846367253No Hit
TGTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG243050.1503840066902785No Hit
CGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTG240180.14860823175013818No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG234580.14514330503766934No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA229700.14212386890251788No Hit
CGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGG229610.1420681825803532No Hit
CCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCC227920.14102251719748313No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT221090.136796544082097No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT214550.1327500046714637No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA209910.12987906539541805No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG209250.12947069903287708No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG207930.12865396630779513No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA207580.1284374083882658No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC206840.12797954307268958No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG205180.12695243979720774No Hit
TTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC204440.12649457448163148No Hit
GTGAGGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT204180.12633370288426687No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA203240.12575209018610245No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA202230.12512716590403217No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT202180.12509622905838513No Hit
CCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGA198400.12275740352746864No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT198000.12250990876229229No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA197970.12249134665490408No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG197790.12237997401057471No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT192860.11932960102977622No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA192810.11929866418412917No Hit
CCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTA191680.118599491472506No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT191450.1184571819825296No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT191420.11843861987514137No Hit
GGCCGGTGGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG189280.1171145228814479No Hit
CGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGG188540.11665665756587167No Hit
TCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCA187050.11573473956558976No Hit
CGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAA181700.1124244970813561No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG177660.10992479995307498Illumina Single End PCR Primer 1 (100% over 50bp)
CCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAG175070.10832227134855814No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC174300.10784584392559368No Hit
TGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCT170870.10572357631420648No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG167700.10376218030018394No Hit
GCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGT167630.10371886871627807No Hit
GCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCTC167000.10332906446112532No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC165750.10255564331994924No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC165530.10241952119910222No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC164090.10152854004446739No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC161740.10007450829905634No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC161630.10000644723863283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)