FastQCFastQC Report
Wed 1 May 2024
EGAF00004925832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18871892
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTAC1390590.7368577564983946No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC751320.39811588578400087No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA735360.389658864092694No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG594470.3150028624580938No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG589300.31226333851423055No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAA588570.3118765198529114No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT581860.3083209675002379No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTA502710.26638028661885094No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA485350.2571814209195347No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT457640.24249820844672065No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT416550.2207250868116456No Hit
CTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAATAAAG408590.21650717373753514No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCC387800.205490790218596No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC379510.2010980139140262No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG369810.19595809471567557No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT326150.17282315943732615No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT298320.15807636033525416No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG297910.15785910601862282No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT287150.15215750492849367No Hit
CCTTAACCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA276360.14644000718105002No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG272610.14445292501673918No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACC272450.14436814284439525No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC243410.1289801785639723No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG233900.12394093819528004No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT226520.12003036049591637No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT223100.11821814156206491No Hit
CCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACCCAAAT221590.11741800981056909No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC215240.11405321734566942No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA214310.11356042096892034No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG209260.11088448365431511No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG203020.1075779789329019No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA195110.10338656028764895No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT194900.10327528368644756No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG193930.10276129176661247No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC192400.10195056224357366No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)