FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004925837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13156531
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA354310.2693035116931659No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA274080.20832239136593075No Hit
GTCCAAGGCTTGTTAGGATAGTTAAGAAAGCTGCCTATTGGCTGGAGGGA225020.17103292653663796No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT199280.15146849880108973No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA195250.1484053813273423No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC190850.14506103470588105No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC177880.13520281296034645No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA143270.10889648646744343No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG138400.10519490282050793No Hit
GTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTAC137020.10414599410741326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA48700.031.7152044
TATCGAA52050.030.124955
CCCGTAT55600.028.6047211
CTTCGCG24350.028.5340446
CGTATCG66950.025.9448853
CCGGTAA9700.025.2996351
CCGTATC70250.024.8614772
ATCGAAG78250.022.3182016
AGTTTAC211000.021.5451994
GTCCAAG112850.021.5371531
CGCGTAT1554.1105068E-521.3132171
CGGTAAT10600.020.3742792
TAGGTAT59500.020.36386394
GTCCGTT7000.020.2258071
TCGACCG15300.019.9488730-31
GAACTCT237600.019.8375439
GTCGGTA9550.019.7669321
GTCCCGT10800.019.2270011
GTCGTTT9150.019.083731
CGACCGT16600.018.95137632-33
Produced by FastQC (version 0.11.5)