FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004925869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15723751
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA643050.4089673004870148No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT485020.30846329225132096No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC477670.30378883511955895No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA468840.2981731267558231No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG418680.26627234175865544No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG361920.23017408505133413No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT344840.21931153704990622No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC300960.19140470998300596No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC269470.17137768208107593No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT267150.16990220717690074No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG263780.1677589526824738No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT253650.1613164695879501No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG250650.15940852790151663No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT234330.14902932512731853No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC221670.14097781121056926No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA216100.13743539947942446No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG207670.1320740833405464No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT207420.1319150882000103No Hit
GTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGCGGC202600.12884966189047387No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA201660.128251840162058No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG195930.12460767154097009No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA190530.12117337650538985No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC176230.11207885446672362No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA169350.10770330819916951No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG164730.10476507800206197No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAA162490.10334048154285831No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT160770.10224659497596979No Hit
CAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGGCCGGTAGC160270.10192860469489753No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)