FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004925997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004925997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3644601
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT209640.5752069979676788No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC152400.4181527689862347No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT131940.36201493661446066No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC121670.3338362690456377No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA111410.30568503932254865No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT98060.26905551526765203No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT93870.257559057905104No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG88260.24216642644832725No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC87250.2393952040291928No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG85340.23415457549399782No Hit
CTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACTATTCCTC82480.22630735161407242No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA78000.21401519672523822No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATATTCCTTACACT68500.18794924327793358No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT65210.17892219203144596No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT57940.1589748781828244No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA57140.156779850524104No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC56970.15631340714662592No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA56920.15617621791795588No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA56290.1544476336367136No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT49940.13702460159562047No Hit
CCAAAATGAACGAAAATCTGTTCGCTTCATTCATTGCCCCCACAATCCTA48060.13186628659762756No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC47970.1316193459860215No Hit
CCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAG45030.12355261934022406No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA43420.11913512617704929No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC42730.11724191482140295No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG42410.11636390375791479No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT42230.1158700225347027No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT41970.11515663854561858No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA40780.11189153490327199No Hit
CAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCAC40770.11186409705753798No Hit
CTCAAACTACGAACGCACTCACAGTCGCATCATAATCCTCTCTCAAGGAC36810.10099871014687205No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)