FastQCFastQC Report
Wed 1 May 2024
EGAF00004926033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004926033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19238199
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC1559400.8105748360332482No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT1335570.6942281863286683No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC702910.36537203924338235No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA678180.35251740560537914No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG626560.32568537210785686No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC601570.31269559068393044No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT585210.30419167615430115No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT552180.28702270935028795No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT519900.27024359192874553No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT485110.25215977857386757No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT455810.2369296626986757No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT449390.2335925519847258No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG444620.23111310991221165No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA431770.22443369049254558No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA416820.21666269280196135No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT408960.21257707127366757No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC385820.2005489183265024No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA378950.19697789798307003No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA378480.19673359237005503No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC375610.1952417687331335No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT372350.19354722341732716No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC370910.19279871260298326No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT358710.18645716264812523No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG356050.18507449683829552No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT327680.17032779419736743No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC315620.16405901612723728No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT303330.15767068424648276No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG275950.14343858279041607No Hit
TTTTAATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTT274100.14247695431365484No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC269120.13988835441404884No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA248000.12891019580367163No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA244700.1271948585208002No Hit
CTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCT242510.12605649832398552No Hit
CCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAA233560.12140429569316755No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA231910.12054662705173183No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA219780.11424146303923773No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA219570.11413230521214589No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA218640.11364889197788212No Hit
CAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAA215410.11196994063737463No Hit
CCCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTAC213500.11097712421001571No Hit
TGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCT211010.10968282426021272No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT210820.10958406241665344No Hit
CGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGG209600.10894990742116764No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG207840.10803506087030289No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA204340.1062157637521059No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG203570.10581551838610256No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC198610.10323731447002912No Hit
AGGACAGAGACGCTGGCTAGGCCGCCCTCCCCACTGTTACCAACATGAAG195500.10162073903071697No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC194780.101246483623545No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG194360.10102816796936137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)