FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004926055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004926055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17923090
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1955911.0912794612982473TruSeq Adapter, Index 13 (97% over 39bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG519980.2901173848928951No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA293750.16389473020556167No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA274420.15310975953365186No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA254160.14180590512015506No Hit
CTTGGATTAAGGCGACAGCGATTTCTAGGATAGTCAGTAGAATTAGAATT213200.11895270291004509No Hit
CTAAGAAGAATTTTATGGAGAAAGGGACGCGGGCGGGGGATATAGGGTCG192970.10766558668176078No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA185000.10321880881031117No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG183970.10264413111801593No Hit
CTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAG180970.10097031259676764No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT180630.10078061316435949No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)