FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004926063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004926063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21931554
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC2072410.9449444394136413No Hit
CGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT2007310.9152611803066942No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1270390.5792521587845532No Hit
CTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC1011670.46128514194662174No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA796170.3630248909858371No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG714070.32559024317200685No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT693290.31611531038794605No Hit
CTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTCACACCT667220.30422832782391984No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC600960.27401615042873845No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT600890.27398423294582774No Hit
CATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCT582890.2657768801973631No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT579780.26435883202804505No Hit
CCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTA558310.2545692840552931No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGC542200.2472237033454173No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAAT528640.24104083094157397No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG527050.24031584811545956No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA520360.23726544867728022No Hit
CATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTAT513320.23405546182454742No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC503120.22940462860041744No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA481420.2195102088981018No Hit
CTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAG471500.21498704560561463No Hit
CGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACC465760.21236981200693758No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA455420.20765514381698627No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT446940.20378856874437626No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT431230.19662537365113297No Hit
AATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTTTGACTAC415670.1895305731641269No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA397390.1811955504840195No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG373700.17039376233895692No Hit
TTTTAATAATCAACACCCTCCTAGCCTTACTACTAATAATTATTACATTT350590.15985643333801153No Hit
GCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTC348080.1587119635936423No Hit
CTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCT344840.15723464009891866No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG339520.1548089113977058No Hit
CCATAATTACCCCCATACTCCTTACACTATTCCTCATCACCCAACTAAAA335980.15319479869050776No Hit
TTTACATAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTA324010.14773690911277879No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG300580.13705367161852736No Hit
CAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAA300090.13683024923815248No Hit
CAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCT296770.13531644862010234No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT296510.1351978979692912No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA294350.13421301563947544No Hit
CACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTA291770.13303662841219552No Hit
TGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCT285330.1301002199844115No Hit
GCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCTC278580.12702246270373727No Hit
AAATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC258980.11808556748874248No Hit
CTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGA248690.11339369750087021No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG238650.10881581852339328No Hit
ATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATCGCTC228920.10437928839880657No Hit
GTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCAC228340.10411482925468939No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC227200.10359503024728663No Hit
CTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCCCATCCTTACC226230.10315274512695269No Hit
AATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGG225920.10301139627406247No Hit
ACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCAT225390.10276973533202434No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA224100.10218154171838438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)