FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004926070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004926070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13156531
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC538610.40938603040573535No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG348340.2647658413908651No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACAC297480.22610823476188366No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA286310.21761815481603775No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA266630.20265980447277476No Hit
AGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCC250900.19070376530105085No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC232150.17645228822096037No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG225570.17145096986432062No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT216450.16451905141256462No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC207170.15746552035639183No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA178020.13530922398921114No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG168020.12770843621316286No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG158580.12053329255257332No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT140830.10704189425008764No Hit
CAAGGATTCAGAGTGCCCCTCCGGCCTCGCCATGAGGCTCTTCCTGTCGC140290.10663145171018104No Hit
CAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCCAAGGCAA139650.10614500129251397No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA139290.10587137293257623No Hit
CAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCCAA132900.10101446954368137No Hit
CTCACTCTCAAGCACGACTAAGAGCCTTCTGAGCCCAGCGACTTCTGAAG132540.10074084118374366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT24650.056.979111
ACCGTTG29600.046.970493
CGCAAAA54550.039.5256771
GACCGTT41250.033.5910152
CCGTTGA43100.032.4764864
GCGATTT26300.032.32631394
GTCGTAA10200.032.2350165
TCGTAAG10550.030.7205036
TCACATG75500.030.4841582
GGTCGTA10600.030.1323534
GTTGACT98400.029.645441
GTAGGTC43600.029.625882
TAACCCC74750.029.22019
AGTAGGT46650.028.194721
GTCTAAC29400.025.8819924
GACTATT116950.025.0217594
CACCCGG38150.024.9937574
CGAGCCG143550.024.34462494
TAGGTCT54250.024.2428323
TCACCCG43100.023.7576943
Produced by FastQC (version 0.11.5)