FastQCFastQC Report
Wed 8 May 2024
EGAF00004926072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004926072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13126935
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC503550.38360058917028234No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG323120.2461503770682189No Hit
AGGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACAC275990.21024709880867087No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA266390.20293389127012512No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA247630.18864266487188366No Hit
AGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCC229490.1748237497938399No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC221600.16881320734809763No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG211440.16107339603647006No Hit
CGCAAAATTAACCCCCTAATAAAACTAATTAACCACTCATTCATCGACCT201600.15357735830946068No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC189700.14451202813147165No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA165560.12612235834183685No Hit
ATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCG158370.12064507061244685No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG149090.11357563665851929No Hit
TGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATT134920.10278103761464501No Hit
CAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCCAAGGCAA133290.1015393159179961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)