FastQCFastQC Report
Mon 8 Aug 2022
EGAF00004926076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004926076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19287127
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC2728661.4147571071627205TruSeq Adapter, Index 6 (100% over 50bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1987631.0305474734521114No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA1222610.6338994916142773No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT906940.4702307399126889No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC813990.42203797382575436No Hit
ACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTT782100.40550362944154406No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC755490.39170686230250884No Hit
CGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT714050.3702210287721961No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC706530.3663220551199772No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC619650.3212764659039161No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA602940.31261265609958394No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG577500.2994225111910136No Hit
GGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC570670.2958812891106073No Hit
CACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACG488030.25303405737930795No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT486070.25201783552314455No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG483340.2506023836520598No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA440600.22844252542123045No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT439870.22806403462786343No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC435510.22580345947843866No Hit
CACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGG376130.1950160850809973No Hit
CGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGG362140.18776254234236128No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC362000.18768995506692104No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC357290.18524791172889565No Hit
CCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTC338630.17557306487378863No Hit
CGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCC311960.16174518890242182No Hit
GACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCG302250.1567107428701019No Hit
ACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG302180.1566744492323818No Hit
ATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTT299580.15532639983134866No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT296770.15386946951715516No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG296440.1536983709393317No Hit
AGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGT288640.1496542227362323No Hit
TACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTT285560.1480573026765469No Hit
GTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGC284130.1473158755059787No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT281950.1461855879312663No Hit
GCCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTT278270.14427757954826553No Hit
CTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGG271450.14074154227324787No Hit
GCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCC271410.1407208030516935No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA266550.13820098763283925No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT265520.1376669526778146No Hit
AGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACC247920.128541695193898No Hit
ACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTC236320.12252732094313477No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG232430.12051043164697366No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC222790.11551227925237387No Hit
GTCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA222170.11519082131828134No Hit
GTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCAT221930.11506638598895523No Hit
CCACGGGCTGGGCCTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGC219830.11397757685735153No Hit
CGCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGC216620.11231325432761448No Hit
GGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGG215510.1117377409294811No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG214910.11142665260616576No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT214880.11141109818999999No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC203790.10566114901405482No Hit
CCGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGG202410.10494564587042952No Hit
GCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCG201090.10426125155913579No Hit
GTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCAC199850.10361833569095076No Hit
CTAGATTTTATGTATACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCA198140.10273173396950204No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG195420.10132146690380583No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)