FastQCFastQC Report
Sat 2 Jul 2022
EGAF00004937002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004937002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97107724
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTTGCATCCAGGTTCCACCTTAATTGGCCTGTGCATCTGACTATGG23498232.419810601265868No Hit
CCCTGGGCTTCACTTACTCTTCATTCAAGGCACACCGGCGATTGGTGAGC17898671.8431767590392705No Hit
TGTACGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCT16199001.6681474276958648No Hit
ATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAAT13730351.4139297508404172No Hit
TAAGCCATTTCGTCTATGCTTCTTCATCAGGGGGCCAGAATTGGACTCTT13716711.4125251251898356No Hit
TAGCATCACTTAATTTGTGTAAGCCTCCTTGGACACAAGCAGTTCTATCA13592241.3997074012361777No Hit
GACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGTGAGATGTGGT13202261.3595478769536395No Hit
TGGGTGGTAGACTGAGCTTAGACAGAGGGTCTTGGCTTGGATACCTATAC12899731.328393815511524No Hit
CGCCTATTGGTAGGGGTGCATCAGGAAGATGCTGGGCTTAATACCTAGGT12737961.3117349964869942No Hit
GGTGTGGCTATCAAGTTCTGCAGCAGTGGTTTGTCTAGTCAAAACTGTGA12403211.27726297034827No Hit
AATGCCAAGAGCTCAGCTTCCCTCCCAGTTGGTGATGGTGAAGTAGGCAG11939291.2294892216812743No Hit
TTTAACGGTCACTTATGTTCTTAATGTTATACCTAAGAAACCATTACCTA11837221.2189782143385421No Hit
GGACACAGTCAGCCAGAAGGCCGAAGGGCCGGCCTCAACGGCACCTCTCC11712491.2061337159956502No Hit
AAGTGAGGTAACCAACAAAAGGGGGTGATATCATATATATCTGTTGTAAG11545711.1889589750862661No Hit
TGACACGCCAAAGGACCCTGCGGTGATCTCCAAGTCCCCATCCATGGCCC10619771.0936071367505227No Hit
CTCATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCA10391161.0700652401244621No Hit
TTTATATCCCTGTAGAACTGAAGCTTGTTGTGACTTCTGCTCCTGTTCTT10260161.0565750670873513No Hit
AGAATGCCAGACATCTGTGGTTGGGCCAGAAGAGGAAGGTTTCTTCCTCA10166501.0469301082579179No Hit
AAAGTTCAACATCGGCACCAAATTTAAAAAGCCCTCTTATATCCTAAGTT9919731.0215181235222854No Hit
GCTGATGGCTTTCTCTTCCGACCTCTCAGAGGGCACTGGCCGGCTCTTCT9587050.9872592627132317No Hit
GGGTGAGGAAAAGTCTGGAGTATTTCCTCTTTATTCTTTATTTGTAATTT9193980.9467815351124901No Hit
TTTTGTTTCCCCAGGCTGAGAAGAAAGCCAAGGTCATTGCAGGAATGAAT8876110.9140477847055709No Hit
GATACTTGGGAAAGGATCCGCCGGCCTTGAACTCCCGCCTCCGCCGCCCC8608660.8865062062416375No Hit
AACAGAATTCTTCACCAGACGCTAGCTTTGTGGTTCCCTGGATGTTATTT8348050.8596690001713974No Hit
TTTTAACTGGCATGAGTCTTTGATCTGGGTAACCCAATTCTCAGGGTCAG8327510.8575538234219144No Hit
TGGTAAATGGTCTGTTTTGGAGAAGTGCACCTGGTGTGAGGGTTTTTGGA8043720.8283295775730466No Hit
TCTTTAACTGCATTTTCTTGGGCTACAGAACTCACAGATTCTTTCTTATC8009530.8248087453887809No Hit
GGACTTCTTTGCCAAGTTCACAGATAATTTCTCTTTCTTCCTGTCTGCCT7785320.8017199538112951No Hit
ATTGGGTGGTAATCTAGAGGTGGCTCCCATGAAGTGGCCATCCATCTCAT7595110.7821324285182505No Hit
AGTTCATAGGGCTGGTGCTTCCATAGATATCTGAAGTGTGTGAAGAGTTT7565290.7790616120299555No Hit
TACGAGGTGCGGCAGAAGTGCCGGAACATTGAGGGTAAGTTTGTTGCCTG7521050.774505846723377No Hit
ACTCCAGTAATTCTGCCGACCAACGAACTGGTCCCTTTGTTCTTCCCTCT7501250.7724668740047907No Hit
CTCTGAAACTAGGTGTGTATTGTTTGAACAAGAAACCTGTATTTTGCATG7262040.747833406125346No Hit
ACCAGGGCTGAGAGTCTCCTCTGCTCACTGGGTCTCCTTCCAGGTGCTTT7004200.7212814502788676No Hit
TTTCACACTCTTCCTCTTTCTCACGGGCTTTTTCAGCCATTTTGGCTTCC6804900.7007578511468356No Hit
ACAAATTGCTGCCAGACTCAAGATTTAAAAAAAGAAGGAAAATCCCATAT6667650.6866240629839084No Hit
TTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCATACCTGGTAG6622450.6819694383940046No Hit
AGTGGGGAGAGTTTGAGTAGAATCTTCGCCTTCACAGGATTGAAGAAATT6387480.6577725990159135No Hit
GTGCCGTGACTCACATTGAGCCCATCCCTTGGCAGGCGGGGACTGTGAAC6325570.651397205025627No Hit
GGATTCCGCTTGGTGAAAACGAGGGGCCTTCAATTCAATCCATCCTGGTT6214010.6399089324758553No Hit
GTTCATTCTAAACACACAATATGCTTCTAATATAAAGTAGAAAGAGGTCA6206610.6391468921668888No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT6161560.6345077143400045No Hit
CCCACCCTAATGCCCTAATGTCTGTCTCTCTGTCCTAGGGCTCCCGGGGC6059870.624035838796922No Hit
GATCTTTTGAGGGTATGACTACACCTTGGACATTCTCTCGAGCTATCTCA6000390.617910682367553No Hit
TGCATCTGACCTGTTGTGCTCACTGCTTAGGACCCTCCAAAGGTTTACCC5996210.6174802325714069No Hit
GGCGTGGGTTGTATTAGGTGGACAGCTTGTCCCAGCTGAGCATGGTCAGG5957500.6134939379281508No Hit
GTGAATGAGGTCCTGTGACTCCCTCTGTTATACATGAAGTCCGACCTCTG5904510.6080371114454295No Hit
ACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAA5766340.5938085831359821No Hit
CCTGCTGTGTGGTTAGACGGCTTCCGGGCAGCCTGGTCTGGCCAGCACTC5578150.5744290742516012No Hit
GCCTTGAGCCTGACCCATCTGCCTTCCAGCCTGTCCTGACAACCCCAACC5569960.5735856809907315No Hit
GCCACCATCTTGATTTGAATTCCCGTTGTCCCAGCCTTAGGCCCTTCAAA5472480.5635473445963989No Hit
AGGAGACCAAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCC5467140.5629974398329014No Hit
TGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAA5233950.5389839020426428No Hit
ACTGTGTAATGATTTCTGCTCCTTGGGGCTCCAGGACGGCCGGGGCTTTG5104040.5256059754834743No Hit
AGCAGAGAAGAGGGGAGAGATTTATTTTATTTTAAGGAATTGGCTTACAT4970900.5118954286272841No Hit
TTACTGCTTTGTGTGTGAAGGCTGGAAAAATTCTGACATATACAAAAGGA4912860.5059185611229031No Hit
AAGGGGCTGGAGTTTCCTGTCAGCCTGTAACTGACCTTGGCACCTGCTTT4745480.4886820331614404No Hit
CACGAGTGCAAATGTTTTGGCTTCTTCTTGTGTAGCTCATCTCATATCTC4713560.48539496199087107No Hit
TGGAATGCTACACTGCTGGGATCCACCTCTGGCCTCACAGGCTGTGTCTG4707620.48478327017529527No Hit
CCACCGAGTCCATGTCCCTACAGTGTGTTCGAGGGCGAGCTCTCCGATAC4667440.48064559725444705No Hit
GGCCCTGTAGAAGTTAGAAGGACTTTTCCTTGAAATAAACGAAGACCCTC4655770.4794438390915227No Hit
GGTAGACGCATGACCAGTGTTGGCTGGTGAATGAATTCGTGCTTATTCCT4645500.47838625071678126No Hit
TATCCTCTCTTCTCCTTTTGTTTCGTTAGGTGGTATGGTCAGGAAAAAGA4561550.469741212346816No Hit
GGAAGAGCTATTTGAATATGCGAGTAGAATGTAAACGAAGAAAGGGGTGC4478040.4611414844817082No Hit
GTTTTCATCTCATGGGCTAATTCTGCCTAAGGAGTTATATTGAGGAGTCT4424810.45565994317815545No Hit
CTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCT4368390.4498499007143861No Hit
AGGAAAAGAAATGATATTAGGCATCCTCTAGGAGCTTCAAAAGATAATTT4248590.4375130859827381No Hit
AATCTGGTATGGTGGAAATGGGTGCAAAGCCTTGAAGTCAGAGGGCCGTG4195430.43203875316859447No Hit
CTGGTCTCTGCTTCTGTCATTACATGTGTATCTCTTTCTGTGTCTCTTCC4181300.4305836680921489No Hit
TCGGAGAAGGGCTGCATACATGTGTAGATGGATTAGGACTCTGGGAAGGT4156380.42801744586249385No Hit
TAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTT4017050.41366946258569504No Hit
AGTACTTCAAACCTTCCCATCTCAGAAATGTACTAATTCTAACACAGTCT3933340.40504913903656115No Hit
CCGGGTTTGAAAAGAGTGAAGTATGCCTCCCCGGCCCGCAGCAGCCGCGG3872460.3987798128190091No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3833550.39477292249172685No Hit
AAACGAACACACAGAATCACACTAGCCTCATCTGGTAGATCACACTAGAC3535930.3641244850924526No Hit
GTTTGAAGAGAGTTATGACGTGTGTGCGTGATTTGGGGAAGGGGCTCTTA3493890.35979527231016145No Hit
CAATTGCTGCCAATGATTATTTAAACTTACCTGATTATTATATGCATCAG3412090.35137163754347694No Hit
GCTGTCTTTGTGAACTCTAAATAATTGAGATACTGAAATACTTAAGAAAT3142820.3236426383549057No Hit
GGCAGTGTCAAACAGTTGTAGTCCTGGCGGGCAGGTGGGAGCACCTCTGC3080340.31720854666514475No Hit
AGTCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATA2940420.30279980612046886No Hit
CAAGAGAAGAATGTGAGCCTGTGTCCGGTGGGACTCCAGACACACAGGCA2910860.2997557640214078No Hit
AAGTGAGGTAACCAACAAAAGGGGATGATATCATATATATCTGTTGTAAG2907730.29943344156639895No Hit
CTCTTGCAGCAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGC2891870.2978002038231274No Hit
TGGCACAGAAATAGAAAGTGATGACCTCAAGGTTACTTACAAATTAGTAG2715630.2796512870593075No Hit
ACGTTATAAATGGTTATTAGGGTTAGCTATGCTGCAGCTGTTACCCGCCT2653760.2732800122058262No Hit
AATCTTCAGCATGTTCCAGAGTAGTACTCAGTTAGCCAGCTGCACTGACC2599790.2677222668713768No Hit
ACTTCCATCCTGACATGCTGATAGTCTCTCCTATAAATGTGAATTAAAAT2554450.2630532253026546No Hit
CTATTGCACCCAGTTCTCCAAGGGCAATGGATTCCCCCTTGTTTCTTCTC2451450.2524464480292011No Hit
CCAGGTGGGAGTTTAGCTTTGGCCTTTAACCCTTCTCATGGGAGAAAGTA2405940.24775990012905666No Hit
ACTTCCATCCTGACATGCTGATAGTCTCTCCTATAAATGTGAATTAAAAA2385450.24564987230058033No Hit
CTTTCTTGTGTAAAGTCAGGACTCACACGACTATTCTGGCTTCCCTTCAT2317320.23863395253708142No Hit
AAAGCGATTATACATCAGGAAGTAAACAAACCTCTTTTGGGTCATCCCCA2285940.23540248971338265No Hit
TTTAACTCTCGCCTCCAAAGACCACGATAATTCCTTCCCCAAAGCCCAGC2191430.22566999922683803No Hit
GAGACTGACTCTCGAGGCTCCCAGCCCCTGCCAGTTGCAAGGCATGGGGT2189810.22550317418622642No Hit
TACCCAAAACCATTTTCCCATCTTCCTTGCTAACAGAACTTGATTTGGGC2157490.22217491164760486No Hit
TAGTGCTATTTTAGAGCAATATACCAAAACATTTGTGAACGACATGATAT2157130.22213783941635784No Hit
CCCTCTAATTTGGGTTTCTCCAGGGAACAGGCACGGTCTCCTGGTACCCA2104200.21668719163884428No Hit
TTTCACATGGGTAATGGACACTATATTACCAGAAATATTAAAGACAAAAT1991900.20512271505817603No Hit
ACACACCTAAAGTCCCACCTACGTGGGAGGCTGAGGCAGGACAACCACTT1888500.19447474641667023No Hit
CCTCTCATTTCCAACAGCAGACCCTACTAGGGGATTAGAGGTCCCTTTCC1807220.18610466042845367No Hit
CAGACCAGGGACTTCTTTAATGGAGAACCTGCCTGGTTTTCCTTCCAGTC1728890.17803836077962243No Hit
ATGGATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGA1672900.17227259903650918No Hit
TGGAACAAACACAGAAGCTACTTGAAGCATTCAATTGCAGTTTACACTTG1576400.16233518149390463No Hit
GAGCATCTTAGCAGGGCTTCAGTCAATTTCCCCAAGTTTTGCACAATGAG1495820.1540371803997795No Hit
AAATTTAGCAGTAAGTGTATAGTGTGTATACAGTCTACAGTGGCATACAG1264160.1301811995923208No Hit
GCAAGACAAGAGGAAGAGAAGATGACAGCCAGAAGAAGTGAGTGCGGTAC1166120.1200851952827151No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)