FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004937010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004937010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70367986
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTTGCATCCAGGTTCCACCTTAATTGGCCTGTGCATCTGACTATGG17745312.5217873934888515No Hit
TGTACGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCT14137112.0090258089808057No Hit
CCCTGGGCTTCACTTACTCTTCATTCAAGGCACACCGGCGATTGGTGAGC13008211.8485977415923203No Hit
GACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGTGAGATGTGGT11987291.7035147204582495No Hit
AAGTGAGGTAACCAACAAAAGGGGGTGATATCATATATATCTGTTGTAAG11969221.7009467913434384No Hit
CGCCTATTGGTAGGGGTGCATCAGGAAGATGCTGGGCTTAATACCTAGGT11014341.565248719780043No Hit
TAGCATCACTTAATTTGTGTAAGCCTCCTTGGACACAAGCAGTTCTATCA10786791.532911571463762No Hit
TGACACGCCAAAGGACCCTGCGGTGATCTCCAAGTCCCCATCCATGGCCC10781931.5322209164832428No Hit
TAAGCCATTTCGTCTATGCTTCTTCATCAGGGGGCCAGAATTGGACTCTT10588931.5047936713720924No Hit
TTTAACGGTCACTTATGTTCTTAATGTTATACCTAAGAAACCATTACCTA10241691.455447367784549No Hit
TGGGTGGTAGACTGAGCTTAGACAGAGGGTCTTGGCTTGGATACCTATAC9623851.3676460770100767No Hit
AGAATGCCAGACATCTGTGGTTGGGCCAGAAGAGGAAGGTTTCTTCCTCA9390351.3344633737279337No Hit
AATGCCAAGAGCTCAGCTTCCCTCCCAGTTGGTGATGGTGAAGTAGGCAG9053031.2865268021170877No Hit
GGACACAGTCAGCCAGAAGGCCGAAGGGCCGGCCTCAACGGCACCTCTCC9038021.284393729841863No Hit
GCTGATGGCTTTCTCTTCCGACCTCTCAGAGGGCACTGGCCGGCTCTTCT8844361.2568726920790372No Hit
TTTTGTTTCCCCAGGCTGAGAAGAAAGCCAAGGTCATTGCAGGAATGAAT8435061.1987070370324369No Hit
ATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAAT8400221.1937559219046001No Hit
TCTTTAACTGCATTTTCTTGGGCTACAGAACTCACAGATTCTTTCTTATC8069821.1468027520355635No Hit
CTCATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCA7815121.1106073150935427No Hit
AGTTCATAGGGCTGGTGCTTCCATAGATATCTGAAGTGTGTGAAGAGTTT7768241.1039451946230208No Hit
TTTTAACTGGCATGAGTCTTTGATCTGGGTAACCCAATTCTCAGGGTCAG7495931.0652471992022052No Hit
GGGTGAGGAAAAGTCTGGAGTATTTCCTCTTTATTCTTTATTTGTAATTT7436581.056812966055331No Hit
TGGTAAATGGTCTGTTTTGGAGAAGTGCACCTGGTGTGAGGGTTTTTGGA7243691.029401353052793No Hit
GGTGTGGCTATCAAGTTCTGCAGCAGTGGTTTGTCTAGTCAAAACTGTGA7184901.0210467015497644No Hit
TTTATATCCCTGTAGAACTGAAGCTTGTTGTGACTTCTGCTCCTGTTCTT6969980.9905044035223631No Hit
ATTGGGTGGTAATCTAGAGGTGGCTCCCATGAAGTGGCCATCCATCTCAT6836790.9715767621941034No Hit
ACTCCAGTAATTCTGCCGACCAACGAACTGGTCCCTTTGTTCTTCCCTCT6805200.9670875048207291No Hit
AAAGTTCAACATCGGCACCAAATTTAAAAAGCCCTCTTATATCCTAAGTT6348910.9022440971949943No Hit
TTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCATACCTGGTAG6092950.8658696015543205No Hit
AGTGGGGAGAGTTTGAGTAGAATCTTCGCCTTCACAGGATTGAAGAAATT6015450.8548560704863715No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT5994520.8518817065476337No Hit
AACAGAATTCTTCACCAGACGCTAGCTTTGTGGTTCCCTGGATGTTATTT5924630.8419496331755183No Hit
TTTCACACTCTTCCTCTTTCTCACGGGCTTTTTCAGCCATTTTGGCTTCC5867790.833872096325167No Hit
TGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAA5821280.8272625565836146No Hit
GATCTTTTGAGGGTATGACTACACCTTGGACATTCTCTCGAGCTATCTCA5732230.8146076541113455No Hit
ACAAATTGCTGCCAGACTCAAGATTTAAAAAAAGAAGGAAAATCCCATAT5686840.8081572776574848No Hit
GGATTCCGCTTGGTGAAAACGAGGGGCCTTCAATTCAATCCATCCTGGTT5665220.8050848577647228No Hit
TACGAGGTGCGGCAGAAGTGCCGGAACATTGAGGGTAAGTTTGTTGCCTG5621740.7989059115604077No Hit
CTCTGAAACTAGGTGTGTATTGTTTGAACAAGAAACCTGTATTTTGCATG5593700.7949211449649844No Hit
CCCACCCTAATGCCCTAATGTCTGTCTCTCTGTCCTAGGGCTCCCGGGGC5556880.7896886518821216No Hit
GTGCCGTGACTCACATTGAGCCCATCCCTTGGCAGGCGGGGACTGTGAAC5496330.7810838866412917No Hit
ACCAGGGCTGAGAGTCTCCTCTGCTCACTGGGTCTCCTTCCAGGTGCTTT5441910.7733502561804171No Hit
GGCGTGGGTTGTATTAGGTGGACAGCTTGTCCCAGCTGAGCATGGTCAGG5406500.7683181383079516No Hit
TTACTGCTTTGTGTGTGAAGGCTGGAAAAATTCTGACATATACAAAAGGA5383520.7650524487087068No Hit
GGACTTCTTTGCCAAGTTCACAGATAATTTCTCTTTCTTCCTGTCTGCCT5325720.7568384861831913No Hit
CCTGCTGTGTGGTTAGACGGCTTCCGGGCAGCCTGGTCTGGCCAGCACTC5310920.7547352570244088No Hit
TGCATCTGACCTGTTGTGCTCACTGCTTAGGACCCTCCAAAGGTTTACCC5290970.7519001609624013No Hit
CACGAGTGCAAATGTTTTGGCTTCTTCTTGTGTAGCTCATCTCATATCTC5263710.7480262402280492No Hit
GCCACCATCTTGATTTGAATTCCCGTTGTCCCAGCCTTAGGCCCTTCAAA5243110.7450987726151491No Hit
GTTCATTCTAAACACACAATATGCTTCTAATATAAAGTAGAAAGAGGTCA5204120.7395579006623836No Hit
GTGAATGAGGTCCTGTGACTCCCTCTGTTATACATGAAGTCCGACCTCTG5063650.7195956979641283No Hit
AGCAGAGAAGAGGGGAGAGATTTATTTTATTTTAAGGAATTGGCTTACAT4962080.7051615773115917No Hit
ACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAA4826720.6859255571134294No Hit
TGGAATGCTACACTGCTGGGATCCACCTCTGGCCTCACAGGCTGTGTCTG4800850.6822491693879089No Hit
AGGAGACCAAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCC4791070.6808593328221728No Hit
GGCCCTGTAGAAGTTAGAAGGACTTTTCCTTGAAATAAACGAAGACCCTC4676610.6645934132603993No Hit
TCGGAGAAGGGCTGCATACATGTGTAGATGGATTAGGACTCTGGGAAGGT4360250.6196354688906401No Hit
GGTAGACGCATGACCAGTGTTGGCTGGTGAATGAATTCGTGCTTATTCCT4322120.6142168116052092No Hit
AATCTGGTATGGTGGAAATGGGTGCAAAGCCTTGAAGTCAGAGGGCCGTG4297660.6107407990900862No Hit
ACTGTGTAATGATTTCTGCTCCTTGGGGCTCCAGGACGGCCGGGGCTTTG4165400.591945320134642No Hit
CTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCT4087060.5808124166009242No Hit
AGGAAAAGAAATGATATTAGGCATCCTCTAGGAGCTTCAAAAGATAATTT4046880.5751024336549863No Hit
TAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTT3976470.5650964630421567No Hit
GGAAGAGCTATTTGAATATGCGAGTAGAATGTAAACGAAGAAAGGGGTGC3860490.5486145361613731No Hit
TATCCTCTCTTCTCCTTTTGTTTCGTTAGGTGGTATGGTCAGGAAAAAGA3852610.5474947087443998No Hit
GCCTTGAGCCTGACCCATCTGCCTTCCAGCCTGTCCTGACAACCCCAACC3838550.5454966410435563No Hit
CTGGTCTCTGCTTCTGTCATTACATGTGTATCTCTTTCTGTGTCTCTTCC3695030.5251010026064978No Hit
AGTACTTCAAACCTTCCCATCTCAGAAATGTACTAATTCTAACACAGTCT3513130.4992511793644343No Hit
AAACGAACACACAGAATCACACTAGCCTCATCTGGTAGATCACACTAGAC3363510.4779886694497694No Hit
GTTTTCATCTCATGGGCTAATTCTGCCTAAGGAGTTATATTGAGGAGTCT3308490.47016977294191714No Hit
CCACCGAGTCCATGTCCCTACAGTGTGTTCGAGGGCGAGCTCTCCGATAC3210000.4561733513305326No Hit
GTTTGAAGAGAGTTATGACGTGTGTGCGTGATTTGGGGAAGGGGCTCTTA3100430.4406023500516272No Hit
CCCTCTAATTTGGGTTTCTCCAGGGAACAGGCACGGTCTCCTGGTACCCA3086660.4386454942734897No Hit
CTCTTGCAGCAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGC2897470.4117596885606475No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2858460.4062159744063159No Hit
ACGTTATAAATGGTTATTAGGGTTAGCTATGCTGCAGCTGTTACCCGCCT2768290.3934019086463552No Hit
CAAGAGAAGAATGTGAGCCTGTGTCCGGTGGGACTCCAGACACACAGGCA2731960.3882390495018573No Hit
CCGGGTTTGAAAAGAGTGAAGTATGCCTCCCCGGCCCGCAGCAGCCGCGG2721240.3867156294625229No Hit
AGTCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATA2581100.36680032309010524No Hit
GGCAGTGTCAAACAGTTGTAGTCCTGGCGGGCAGGTGGGAGCACCTCTGC2548060.3621050061032015No Hit
TTTAACTCTCGCCTCCAAAGACCACGATAATTCCTTCCCCAAAGCCCAGC2505660.3560795387834462No Hit
ACTTCCATCCTGACATGCTGATAGTCTCTCCTATAAATGTGAATTAAAAT2489000.35371198487903294No Hit
CTTTCTTGTGTAAAGTCAGGACTCACACGACTATTCTGGCTTCCCTTCAT2480020.3524358363759338No Hit
CAATTGCTGCCAATGATTATTTAAACTTACCTGATTATTATATGCATCAG2444680.34741366620894903No Hit
AATCTTCAGCATGTTCCAGAGTAGTACTCAGTTAGCCAGCTGCACTGACC2434290.3459371424954524No Hit
ACTTCCATCCTGACATGCTGATAGTCTCTCCTATAAATGTGAATTAAAAA2420610.3439930766243615No Hit
AAGGGGCTGGAGTTTCCTGTCAGCCTGTAACTGACCTTGGCACCTGCTTT2362860.33578621960276084No Hit
CTATTGCACCCAGTTCTCCAAGGGCAATGGATTCCCCCTTGTTTCTTCTC2325230.330438617356478No Hit
CCAGGTGGGAGTTTAGCTTTGGCCTTTAACCCTTCTCATGGGAGAAAGTA2322380.33003360363333406No Hit
ATGGATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGA2107190.2994529358847928No Hit
GCTGTCTTTGTGAACTCTAAATAATTGAGATACTGAAATACTTAAGAAAT1997410.2838520914894452No Hit
AAGTGAGGTAACCAACAAAAGGGGATGATATCATATATATCTGTTGTAAG1949770.27708196735941826No Hit
GATACTTGGGAAAGGATCCGCCGGCCTTGAACTCCCGGCTCCGCCGCCCC1939260.2755883904365261No Hit
TACCCAAAACCATTTTCCCATCTTCCTTGCTAACAGAACTTGATTTGGGC1922800.273249258547772No Hit
AAAGCGATTATACATCAGGAAGTAAACAAACCTCTTTTGGGTCATCCCCA1899860.2699892533516591No Hit
GAGACTGACTCTCGAGGCTCCCAGCCCCTGCCAGTTGCAAGGCATGGGGT1876320.26664398210856854No Hit
CCTCTCATTTCCAACAGCAGACCCTACTAGGGGATTAGAGGTCCCTTTCC1852670.26328307875686535No Hit
TGGCACAGAAATAGAAAGTGATGACCTCAAGGTTACTTACAAATTAGTAG1640870.233184164173748No Hit
TAGTGCTATTTTAGAGCAATATACCAAAACATTTGTGAACGACATGATAT1596670.22690289871305966No Hit
GATACTTGGGAAAGGATCCGCCGGCCTTGAACTCCCGCCTCCGCCGCCCC1496110.21261230923960225No Hit
GAGCATCTTAGCAGGGCTTCAGTCAATTTCCCCAAGTTTTGCACAATGAG1424910.20249407166491878No Hit
TTTCACATGGGTAATGGACACTATATTACCAGAAATATTAAAGACAAAAT1416380.2012818726970529No Hit
CTGTCCTCAGCCCAACTTACCAATGTGCTGCCATTCTGCATGTTGTGCAA1352020.19213566805791485No Hit
CAGACCAGGGACTTCTTTAATGGAGAACCTGCCTGGTTTTCCTTCCAGTC1270120.18049685264546295No Hit
TGGAACAAACACAGAAGCTACTTGAAGCATTCAATTGCAGTTTACACTTG1187520.1687585601782038No Hit
AAATTTAGCAGTAAGTGTATAGTGTGTATACAGTCTACAGTGGCATACAG1075190.15279533508320103No Hit
ACACACCTAAAGTCCCACCTACGTGGGAGGCTGAGGCAGGACAACCACTT994290.14129862974904525No Hit
GCAAGACAAGAGGAAGAGAAGATGACAGCCAGAAGAAGTGAGTGCGGTAC976470.13876622815380849No Hit
ACGTTATAAATGGTTATTAGGGTTAGCTATGCTGCAGCTGTTACCCACCT940140.13360336900931055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)