FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004937016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004937016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59543762
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTTGCATCCAGGTTCCACCTTAATTGGCCTGTGCATCTGACTATGG15821632.6571431613608825No Hit
CCCTGGGCTTCACTTACTCTTCATTCAAGGCACACCGGCGATTGGTGAGC13415832.2531041958685782No Hit
TGTACGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCT10346821.7376832857823126No Hit
ATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAAT9121671.5319270555998796No Hit
GGACACAGTCAGCCAGAAGGCCGAAGGGCCGGCCTCAACGGCACCTCTCC9026371.5159220205132486No Hit
TAAGCCATTTCGTCTATGCTTCTTCATCAGGGGGCCAGAATTGGACTCTT8946661.5025352277875892No Hit
GACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGTGAGATGTGGT8562471.4380129357631115No Hit
CGCCTATTGGTAGGGGTGCATCAGGAAGATGCTGGGCTTAATACCTAGGT8437861.4170854706828904No Hit
AGAATGCCAGACATCTGTGGTTGGGCCAGAAGAGGAAGGTTTCTTCCTCA7818101.313000680071239No Hit
TGGGTGGTAGACTGAGCTTAGACAGAGGGTCTTGGCTTGGATACCTATAC7667631.2877301907796823No Hit
TGACACGCCAAAGGACCCTGCGGTGATCTCCAAGTCCCCATCCATGGCCC7629191.2812744347594296No Hit
TAGCATCACTTAATTTGTGTAAGCCTCCTTGGACACAAGCAGTTCTATCA7602421.276778581776543No Hit
AAGTGAGGTAACCAACAAAAGGGGGTGATATCATATATATCTGTTGTAAG7486721.2573474951078838No Hit
CTCATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCA7267811.2205829386460332No Hit
GCTGATGGCTTTCTCTTCCGACCTCTCAGAGGGCACTGGCCGGCTCTTCT6990501.1740104698121023No Hit
AATGCCAAGAGCTCAGCTTCCCTCCCAGTTGGTGATGGTGAAGTAGGCAG6763651.1359124403325407No Hit
GGTGTGGCTATCAAGTTCTGCAGCAGTGGTTTGTCTAGTCAAAACTGTGA6719651.1285229173124802No Hit
TTTAACGGTCACTTATGTTCTTAATGTTATACCTAAGAAACCATTACCTA6681661.122142735959478No Hit
TTTATATCCCTGTAGAACTGAAGCTTGTTGTGACTTCTGCTCCTGTTCTT6271161.0532018450564142No Hit
TTTTGTTTCCCCAGGCTGAGAAGAAAGCCAAGGTCATTGCAGGAATGAAT5790670.9725065742403042No Hit
TTTTAACTGGCATGAGTCTTTGATCTGGGTAACCCAATTCTCAGGGTCAG5787840.9720312935551503No Hit
AGTTCATAGGGCTGGTGCTTCCATAGATATCTGAAGTGTGTGAAGAGTTT5782760.9711781395337432No Hit
AAAGTTCAACATCGGCACCAAATTTAAAAAGCCCTCTTATATCCTAAGTT5700270.95732446330818No Hit
GGGTGAGGAAAAGTCTGGAGTATTTCCTCTTTATTCTTTATTTGTAATTT5549730.9320422179572732No Hit
ATTGGGTGGTAATCTAGAGGTGGCTCCCATGAAGTGGCCATCCATCTCAT5476860.919804160173823No Hit
TGGTAAATGGTCTGTTTTGGAGAAGTGCACCTGGTGTGAGGGTTTTTGGA5072310.8518625343155175No Hit
ACTCCAGTAATTCTGCCGACCAACGAACTGGTCCCTTTGTTCTTCCCTCT4992630.838480779901008No Hit
TACGAGGTGCGGCAGAAGTGCCGGAACATTGAGGGTAAGTTTGTTGCCTG4980470.8364385844481912No Hit
TCTTTAACTGCATTTTCTTGGGCTACAGAACTCACAGATTCTTTCTTATC4977920.8360103280004378No Hit
TTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCATACCTGGTAG4629860.7775558420376596No Hit
TTTCACACTCTTCCTCTTTCTCACGGGCTTTTTCAGCCATTTTGGCTTCC4623110.7764222220289003No Hit
GTTCATTCTAAACACACAATATGCTTCTAATATAAAGTAGAAAGAGGTCA4616690.7753440234427916No Hit
GGACTTCTTTGCCAAGTTCACAGATAATTTCTCTTTCTTCCTGTCTGCCT4601020.77271234558542No Hit
AACAGAATTCTTCACCAGACGCTAGCTTTGTGGTTCCCTGGATGTTATTT4597730.7721598107959655No Hit
GCCTTGAGCCTGACCCATCTGCCTTCCAGCCTGTCCTGACAACCCCAACC4447610.7469481018011593No Hit
CTCTGAAACTAGGTGTGTATTGTTTGAACAAGAAACCTGTATTTTGCATG4427750.743612739819832No Hit
ACAAATTGCTGCCAGACTCAAGATTTAAAAAAAGAAGGAAAATCCCATAT4380330.7356488493286669No Hit
GGATTCCGCTTGGTGAAAACGAGGGGCCTTCAATTCAATCCATCCTGGTT4227490.709980333456257No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT4135760.694574857396481No Hit
ACCAGGGCTGAGAGTCTCCTCTGCTCACTGGGTCTCCTTCCAGGTGCTTT4135410.6945160770997305No Hit
GGCGTGGGTTGTATTAGGTGGACAGCTTGTCCCAGCTGAGCATGGTCAGG4111840.6905576439728481No Hit
CCCACCCTAATGCCCTAATGTCTGTCTCTCTGTCCTAGGGCTCCCGGGGC4087470.6864648558819646No Hit
GATACTTGGGAAAGGATCCGCCGGCCTTGAACTCCCGCCTCCGCCGCCCC4038240.678196987284747No Hit
GATCTTTTGAGGGTATGACTACACCTTGGACATTCTCTCGAGCTATCTCA4030550.6769055001932864No Hit
AGGAGACCAAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCC3996300.6711534282969894No Hit
ACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAA3888940.6531229921280418No Hit
TGCATCTGACCTGTTGTGCTCACTGCTTAGGACCCTCCAAAGGTTTACCC3887010.6527988607773892No Hit
GCCACCATCTTGATTTGAATTCCCGTTGTCCCAGCCTTAGGCCCTTCAAA3875950.650941403400074No Hit
CCTGCTGTGTGGTTAGACGGCTTCCGGGCAGCCTGGTCTGGCCAGCACTC3848820.6463850906833868No Hit
TGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAA3717980.6244113363210071No Hit
ACTTCCATCCTGACATGCTGATAGTCTCTCCTATAAATGTGAATTAAAAT3693060.6202261791923729No Hit
AGTGGGGAGAGTTTGAGTAGAATCTTCGCCTTCACAGGATTGAAGAAATT3553280.5967510081072808No Hit
TTACTGCTTTGTGTGTGAAGGCTGGAAAAATTCTGACATATACAAAAGGA3538240.5942251347840601No Hit
CACGAGTGCAAATGTTTTGGCTTCTTCTTGTGTAGCTCATCTCATATCTC3528760.5926330284606471No Hit
GTGCCGTGACTCACATTGAGCCCATCCCTTGGCAGGCGGGGACTGTGAAC3508270.589191861945169No Hit
ACTGTGTAATGATTTCTGCTCCTTGGGGCTCCAGGACGGCCGGGGCTTTG3238850.543944468943699No Hit
CTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCT3230550.5425505361921875No Hit
GTGAATGAGGTCCTGTGACTCCCTCTGTTATACATGAAGTCCGACCTCTG3147380.5285826582472233No Hit
TATCCTCTCTTCTCCTTTTGTTTCGTTAGGTGGTATGGTCAGGAAAAAGA3107590.5219001782252186No Hit
TGGAATGCTACACTGCTGGGATCCACCTCTGGCCTCACAGGCTGTGTCTG3104940.521455127406965No Hit
GTTTTCATCTCATGGGCTAATTCTGCCTAAGGAGTTATATTGAGGAGTCT3099500.5205415136517575No Hit
GGCCCTGTAGAAGTTAGAAGGACTTTTCCTTGAAATAAACGAAGACCCTC3090540.5190367380549452No Hit
CTGGTCTCTGCTTCTGTCATTACATGTGTATCTCTTTCTGTGTCTCTTCC3045770.5115178983820338No Hit
AGGAAAAGAAATGATATTAGGCATCCTCTAGGAGCTTCAAAAGATAATTT2994780.5029544488640136No Hit
GGAAGAGCTATTTGAATATGCGAGTAGAATGTAAACGAAGAAAGGGGTGC2912500.48913604081650064No Hit
CCACCGAGTCCATGTCCCTACAGTGTGTTCGAGGGCGAGCTCTCCGATAC2907830.48835174371414425No Hit
GGTAGACGCATGACCAGTGTTGGCTGGTGAATGAATTCGTGCTTATTCCT2893840.486002211281175No Hit
TCGGAGAAGGGCTGCATACATGTGTAGATGGATTAGGACTCTGGGAAGGT2817250.4731394029151198No Hit
AGTACTTCAAACCTTCCCATCTCAGAAATGTACTAATTCTAACACAGTCT2730830.4586257079289011No Hit
AATCTGGTATGGTGGAAATGGGTGCAAAGCCTTGAAGTCAGAGGGCCGTG2705650.4543968854369665No Hit
AGCAGAGAAGAGGGGAGAGATTTATTTTATTTTAAGGAATTGGCTTACAT2630370.44175408332446314No Hit
TAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTT2625190.440884134932556No Hit
CCGGGTTTGAAAAGAGTGAAGTATGCCTCCCCGGCCCGCAGCAGCCGCGG2517390.4227798035334079No Hit
AAACGAACACACAGAATCACACTAGCCTCATCTGGTAGATCACACTAGAC2473130.41534661515004706No Hit
GTTTGAAGAGAGTTATGACGTGTGTGCGTGATTTGGGGAAGGGGCTCTTA2376660.39914508592856457No Hit
CAATTGCTGCCAATGATTATTTAAACTTACCTGATTATTATATGCATCAG2359960.39634042605504166No Hit
GCTGTCTTTGTGAACTCTAAATAATTGAGATACTGAAATACTTAAGAAAT2353620.39527566296533295No Hit
AAGGGGCTGGAGTTTCCTGTCAGCCTGTAACTGACCTTGGCACCTGCTTT2267180.38075860910501425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2244330.3769210954457329No Hit
CAAGAGAAGAATGTGAGCCTGTGTCCGGTGGGACTCCAGACACACAGGCA2178320.36583513147859215No Hit
GGCAGTGTCAAACAGTTGTAGTCCTGGCGGGCAGGTGGGAGCACCTCTGC2098890.352495362990333No Hit
CTCTTGCAGCAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGC2059840.34593716131002944No Hit
AGTCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATA2016720.3386954287503702No Hit
ACGTTATAAATGGTTATTAGGGTTAGCTATGCTGCAGCTGTTACCCGCCT1954870.3283081105960352No Hit
TGGCACAGAAATAGAAAGTGATGACCTCAAGGTTACTTACAAATTAGTAG1869130.31390861732921743No Hit
AATCTTCAGCATGTTCCAGAGTAGTACTCAGTTAGCCAGCTGCACTGACC1809500.30389413419998557No Hit
TTTAACTCTCGCCTCCAAAGACCACGATAATTCCTTCCCCAAAGCCCAGC1674180.2811679920391997No Hit
CCAGGTGGGAGTTTAGCTTTGGCCTTTAACCCTTCTCATGGGAGAAAGTA1659190.2786505159012291No Hit
CTATTGCACCCAGTTCTCCAAGGGCAATGGATTCCCCCTTGTTTCTTCTC1629560.2736743439220384No Hit
AAAGCGATTATACATCAGGAAGTAAACAAACCTCTTTTGGGTCATCCCCA1622990.27257095378017937No Hit
CTTTCTTGTGTAAAGTCAGGACTCACACGACTATTCTGGCTTCCCTTCAT1616380.2714608458901203No Hit
CCCTCTAATTTGGGTTTCTCCAGGGAACAGGCACGGTCTCCTGGTACCCA1593210.26756959024523846No Hit
AAGTGAGGTAACCAACAAAAGGGGATGATATCATATATATCTGTTGTAAG1582110.26570541511972323No Hit
GAGACTGACTCTCGAGGCTCCCAGCCCCTGCCAGTTGCAAGGCATGGGGT1545580.25957043157602305No Hit
TACCCAAAACCATTTTCCCATCTTCCTTGCTAACAGAACTTGATTTGGGC1528470.2566969147834495No Hit
ACACACCTAAAGTCCCACCTACGTGGGAGGCTGAGGCAGGACAACCACTT1514350.2543255496688301No Hit
TAGTGCTATTTTAGAGCAATATACCAAAACATTTGTGAACGACATGATAT1451370.24374845512784363No Hit
CCTCTCATTTCCAACAGCAGACCCTACTAGGGGATTAGAGGTCCCTTTCC1362510.22882497750142156No Hit
CAGACCAGGGACTTCTTTAATGGAGAACCTGCCTGGTTTTCCTTCCAGTC1321170.2218821847366648No Hit
TTTCACATGGGTAATGGACACTATATTACCAGAAATATTAAAGACAAAAT1297140.21784649750548174No Hit
ATGGATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGA1235870.2075565867000476No Hit
GAGCATCTTAGCAGGGCTTCAGTCAATTTCCCCAAGTTTTGCACAATGAG1116520.18751250550813367No Hit
TGGAACAAACACAGAAGCTACTTGAAGCATTCAATTGCAGTTTACACTTG1056180.17737878234835078No Hit
CTGTCCTCAGCCCAACTTACCAATGTGCTGCCATTCTGCATGTTGTGCAA979570.16451261510819556No Hit
GCAAGACAAGAGGAAGAGAAGATGACAGCCAGAAGAAGTGAGTGCGGTAC851250.1429620788824193No Hit
AAATTTAGCAGTAAGTGTATAGTGTGTATACAGTCTACAGTGGCATACAG807120.13555072318070868No Hit
ATTGGGTGGTAATCTAGAGGTGGCCCCCATGAAGTGGCCATCCATCTCAT698740.1173489844326598No Hit
ACGTTATAAATGGTTATTAGGGTTAGCTATGCTGCAGCTGTTACCCACCT669900.11250548798042018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)