FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004937022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004937022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74053582
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTTGCATCCAGGTTCCACCTTAATTGGCCTGTGCATCTGACTATGG16916992.2844256203568922No Hit
CCCTGGGCTTCACTTACTCTTCATTCAAGGCACACCGGCGATTGGTGAGC16316022.203272219836712No Hit
GGACACAGTCAGCCAGAAGGCCGAAGGGCCGGCCTCAACGGCACCTCTCC12910891.7434524639199762No Hit
TGACACGCCAAAGGACCCTGCGGTGATCTCCAAGTCCCCATCCATGGCCC12117521.6363178758861385No Hit
TGTACGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCT12045641.6266113906549449No Hit
TAAGCCATTTCGTCTATGCTTCTTCATCAGGGGGCCAGAATTGGACTCTT11262151.5208109717096467No Hit
GACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGTGAGATGTGGT11238381.5176011337304385No Hit
TTTAACGGTCACTTATGTTCTTAATGTTATACCTAAGAAACCATTACCTA11039781.4907827146025157No Hit
CGCCTATTGGTAGGGGTGCATCAGGAAGATGCTGGGCTTAATACCTAGGT9995541.349771304783069No Hit
AGAATGCCAGACATCTGTGGTTGGGCCAGAAGAGGAAGGTTTCTTCCTCA9938431.342059321316827No Hit
ATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAAT9441771.2749916675198778No Hit
TAGCATCACTTAATTTGTGTAAGCCTCCTTGGACACAAGCAGTTCTATCA9347951.2623224626730414No Hit
GCTGATGGCTTTCTCTTCCGACCTCTCAGAGGGCACTGGCCGGCTCTTCT9301621.256066181916764No Hit
CTCATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCA9247671.248780916499083No Hit
AAGTGAGGTAACCAACAAAAGGGGGTGATATCATATATATCTGTTGTAAG8675101.1714625769216673No Hit
TTTTGTTTCCCCAGGCTGAGAAGAAAGCCAAGGTCATTGCAGGAATGAAT8592391.1602936371126518No Hit
AATGCCAAGAGCTCAGCTTCCCTCCCAGTTGGTGATGGTGAAGTAGGCAG8415951.1364676458189422No Hit
TGGGTGGTAGACTGAGCTTAGACAGAGGGTCTTGGCTTGGATACCTATAC8396231.1338047091361496No Hit
TTTTAACTGGCATGAGTCTTTGATCTGGGTAACCCAATTCTCAGGGTCAG7802661.0536505850588023No Hit
GGTGTGGCTATCAAGTTCTGCAGCAGTGGTTTGTCTAGTCAAAACTGTGA7689271.0383386991327441No Hit
GCCTTGAGCCTGACCCATCTGCCTTCCAGCCTGTCCTGACAACCCCAACC7256500.9798985820834433No Hit
TCTTTAACTGCATTTTCTTGGGCTACAGAACTCACAGATTCTTTCTTATC7182910.9699611829715408No Hit
GGGTGAGGAAAAGTCTGGAGTATTTCCTCTTTATTCTTTATTTGTAATTT7026290.948811632096338No Hit
TGGTAAATGGTCTGTTTTGGAGAAGTGCACCTGGTGTGAGGGTTTTTGGA7013280.9470547960799519No Hit
AAAGTTCAACATCGGCACCAAATTTAAAAAGCCCTCTTATATCCTAAGTT7001610.945478910122133No Hit
TACGAGGTGCGGCAGAAGTGCCGGAACATTGAGGGTAAGTTTGTTGCCTG6948600.9383205798201631No Hit
GTTCATTCTAAACACACAATATGCTTCTAATATAAAGTAGAAAGAGGTCA6879540.9289948999361031No Hit
ACTCCAGTAATTCTGCCGACCAACGAACTGGTCCCTTTGTTCTTCCCTCT6779190.9154439011471451No Hit
AGTTCATAGGGCTGGTGCTTCCATAGATATCTGAAGTGTGTGAAGAGTTT6561400.8860341151357134No Hit
TTTATATCCCTGTAGAACTGAAGCTTGTTGTGACTTCTGCTCCTGTTCTT6419640.8668912193876049No Hit
TTTCACACTCTTCCTCTTTCTCACGGGCTTTTTCAGCCATTTTGGCTTCC6389470.8628171423227036No Hit
AACAGAATTCTTCACCAGACGCTAGCTTTGTGGTTCCCTGGATGTTATTT6329210.8546797911814717No Hit
GGACTTCTTTGCCAAGTTCACAGATAATTTCTCTTTCTTCCTGTCTGCCT6312860.8524719303922395No Hit
CCCACCCTAATGCCCTAATGTCTGTCTCTCTGTCCTAGGGCTCCCGGGGC6247940.8437053051667371No Hit
GGCGTGGGTTGTATTAGGTGGACAGCTTGTCCCAGCTGAGCATGGTCAGG6167030.8327794326005729No Hit
ACAAATTGCTGCCAGACTCAAGATTTAAAAAAAGAAGGAAAATCCCATAT6100860.8238440106786461No Hit
TTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCATACCTGGTAG6087730.8220709701794034No Hit
ACCAGGGCTGAGAGTCTCCTCTGCTCACTGGGTCTCCTTCCAGGTGCTTT6060690.8184195600423488No Hit
TGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAA6021910.8131828113324755No Hit
CTCTGAAACTAGGTGTGTATTGTTTGAACAAGAAACCTGTATTTTGCATG5990480.808938587197578No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT5949310.8033790992041412No Hit
CCTGCTGTGTGGTTAGACGGCTTCCGGGCAGCCTGGTCTGGCCAGCACTC5940700.8022164275591692No Hit
ATTGGGTGGTAATCTAGAGGTGGCTCCCATGAAGTGGCCATCCATCTCAT5771940.7794275231682918No Hit
GGATTCCGCTTGGTGAAAACGAGGGGCCTTCAATTCAATCCATCCTGGTT5769100.7790440170740154No Hit
ACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAA5758170.7775680587604796No Hit
GTGCCGTGACTCACATTGAGCCCATCCCTTGGCAGGCGGGGACTGTGAAC5562070.7510872330254058No Hit
TGCATCTGACCTGTTGTGCTCACTGCTTAGGACCCTCCAAAGGTTTACCC5433450.7337187281501116No Hit
GCCACCATCTTGATTTGAATTCCCGTTGTCCCAGCCTTAGGCCCTTCAAA5387060.727454345152406No Hit
TTACTGCTTTGTGTGTGAAGGCTGGAAAAATTCTGACATATACAAAAGGA5360760.7239028626596348No Hit
GATCTTTTGAGGGTATGACTACACCTTGGACATTCTCTCGAGCTATCTCA5340250.7211332464647017No Hit
AGTGGGGAGAGTTTGAGTAGAATCTTCGCCTTCACAGGATTGAAGAAATT5337180.7207186817782831No Hit
GATACTTGGGAAAGGATCCGCCGGCCTTGAACTCCCGCCTCCGCCGCCCC5326570.7192859354190322No Hit
CACGAGTGCAAATGTTTTGGCTTCTTCTTGTGTAGCTCATCTCATATCTC5272240.7119493558056381No Hit
TGGAATGCTACACTGCTGGGATCCACCTCTGGCCTCACAGGCTGTGTCTG5162320.6971060495088542No Hit
AGGAGACCAAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCC5115460.6907781989532931No Hit
CTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCT5110700.6901354211333086No Hit
CTGGTCTCTGCTTCTGTCATTACATGTGTATCTCTTTCTGTGTCTCTTCC4723100.637794941506003No Hit
AGGAAAAGAAATGATATTAGGCATCCTCTAGGAGCTTCAAAAGATAATTT4661940.6295360567433457No Hit
ACTGTGTAATGATTTCTGCTCCTTGGGGCTCCAGGACGGCCGGGGCTTTG4607170.6221400606928102No Hit
GGTAGACGCATGACCAGTGTTGGCTGGTGAATGAATTCGTGCTTATTCCT4605730.6219456068985292No Hit
TATCCTCTCTTCTCCTTTTGTTTCGTTAGGTGGTATGGTCAGGAAAAAGA4598170.620924724478554No Hit
TAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTT4596750.6207329714314157No Hit
CCGGGTTTGAAAAGAGTGAAGTATGCCTCCCCGGCCCGCAGCAGCCGCGG4361190.5889235715836135No Hit
AATCTGGTATGGTGGAAATGGGTGCAAAGCCTTGAAGTCAGAGGGCCGTG4312990.5824147709694854No Hit
TCGGAGAAGGGCTGCATACATGTGTAGATGGATTAGGACTCTGGGAAGGT4284430.5785581040495786No Hit
GTGAATGAGGTCCTGTGACTCCCTCTGTTATACATGAAGTCCGACCTCTG4213890.5690325688769519No Hit
AGTACTTCAAACCTTCCCATCTCAGAAATGTACTAATTCTAACACAGTCT4176060.5639241056563611No Hit
GGCCCTGTAGAAGTTAGAAGGACTTTTCCTTGAAATAAACGAAGACCCTC4154550.5610194521042885No Hit
GGAAGAGCTATTTGAATATGCGAGTAGAATGTAAACGAAGAAAGGGGTGC4134530.5583160042143539No Hit
GTTTGAAGAGAGTTATGACGTGTGTGCGTGATTTGGGGAAGGGGCTCTTA4125790.5571357777129539No Hit
AGCAGAGAAGAGGGGAGAGATTTATTTTATTTTAAGGAATTGGCTTACAT4105280.5543661615180208No Hit
GTTTTCATCTCATGGGCTAATTCTGCCTAAGGAGTTATATTGAGGAGTCT3985610.538206240989126No Hit
AAACGAACACACAGAATCACACTAGCCTCATCTGGTAGATCACACTAGAC3740080.5050505186906421No Hit
GCTGTCTTTGTGAACTCTAAATAATTGAGATACTGAAATACTTAAGAAAT3639380.4914522568266853No Hit
CCACCGAGTCCATGTCCCTACAGTGTGTTCGAGGGCGAGCTCTCCGATAC3514130.4745388278449515No Hit
CTCTTGCAGCAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGC3483730.4704336921879079No Hit
CAAGAGAAGAATGTGAGCCTGTGTCCGGTGGGACTCCAGACACACAGGCA3475880.4693736489343622No Hit
ACGTTATAAATGGTTATTAGGGTTAGCTATGCTGCAGCTGTTACCCGCCT3246590.43841093331582526No Hit
CAATTGCTGCCAATGATTATTTAAACTTACCTGATTATTATATGCATCAG3199390.4320371700588366No Hit
AGTCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATA3190320.4308123812295805No Hit
AAGGGGCTGGAGTTTCCTGTCAGCCTGTAACTGACCTTGGCACCTGCTTT3167610.4277456828489404No Hit
GGCAGTGTCAAACAGTTGTAGTCCTGGCGGGCAGGTGGGAGCACCTCTGC3127050.42226856764335857No Hit
TGGCACAGAAATAGAAAGTGATGACCTCAAGGTTACTTACAAATTAGTAG2895050.39093990078697344No Hit
GAGACTGACTCTCGAGGCTCCCAGCCCCTGCCAGTTGCAAGGCATGGGGT2886610.3898001854927152No Hit
TTTAACTCTCGCCTCCAAAGACCACGATAATTCCTTCCCCAAAGCCCAGC2765900.3734998261123952No Hit
CCAGGTGGGAGTTTAGCTTTGGCCTTTAACCCTTCTCATGGGAGAAAGTA2704540.36521393387830986No Hit
ACTTCCATCCTGACATGCTGATAGTCTCTCCTATAAATGTGAATTAAAAT2665230.3599056153691526No Hit
ACTTCCATCCTGACATGCTGATAGTCTCTCCTATAAATGTGAATTAAAAA2660780.3593046991298814No Hit
AATCTTCAGCATGTTCCAGAGTAGTACTCAGTTAGCCAGCTGCACTGACC2615070.3531321415350307No Hit
CCCTCTAATTTGGGTTTCTCCAGGGAACAGGCACGGTCTCCTGGTACCCA2595880.35054077465152195No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2543680.34349182460883526No Hit
AAAGCGATTATACATCAGGAAGTAAACAAACCTCTTTTGGGTCATCCCCA2526860.34122049626174733No Hit
CTATTGCACCCAGTTCTCCAAGGGCAATGGATTCCCCCTTGTTTCTTCTC2484650.33552056941688524No Hit
CTTTCTTGTGTAAAGTCAGGACTCACACGACTATTCTGGCTTCCCTTCAT2479060.3347657105904749No Hit
TACCCAAAACCATTTTCCCATCTTCCTTGCTAACAGAACTTGATTTGGGC2440390.32954381598988686No Hit
ATGGATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGA2112820.285309628911671No Hit
ACACACCTAAAGTCCCACCTACGTGGGAGGCTGAGGCAGGACAACCACTT2040650.2755639828469067No Hit
CAGACCAGGGACTTCTTTAATGGAGAACCTGCCTGGTTTTCCTTCCAGTC2001640.27029617554489127No Hit
CCTCTCATTTCCAACAGCAGACCCTACTAGGGGATTAGAGGTCCCTTTCC1982280.26768185231066877No Hit
TAGTGCTATTTTAGAGCAATATACCAAAACATTTGTGAACGACATGATAT1959020.2645408833836019No Hit
TTTCACATGGGTAATGGACACTATATTACCAGAAATATTAAAGACAAAAT1879020.2537378948124346No Hit
GAGCATCTTAGCAGGGCTTCAGTCAATTTCCCCAAGTTTTGCACAATGAG1690930.22833871830804892No Hit
GCAAGACAAGAGGAAGAGAAGATGACAGCCAGAAGAAGTGAGTGCGGTAC1605620.2168186813704704No Hit
TGGAACAAACACAGAAGCTACTTGAAGCATTCAATTGCAGTTTACACTTG1534260.20718241556498912No Hit
CTGTCCTCAGCCCAACTTACCAATGTGCTGCCATTCTGCATGTTGTGCAA1464920.1978189252209299No Hit
AAGTGAGGTAACCAACAAAAGGGGATGATATCATATATATCTGTTGTAAG1461550.19736384932736947No Hit
AAATTTAGCAGTAAGTGTATAGTGTGTATACAGTCTACAGTGGCATACAG1266050.17096404600657938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)