FastQCFastQC Report
Sat 2 Jul 2022
EGAF00004937024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004937024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60748358
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCTTGCATCCAGGTTCCACCTTAATTGGCCTGTGCATCTGACTATGG15065432.4799732035555593No Hit
CCCTGGGCTTCACTTACTCTTCATTCAAGGCACACCGGCGATTGGTGAGC13615952.241369223510535No Hit
TGTACGTGATGGGGCTGACTTTTCCTTTTCATTTTTATTTGAGCTGTTCT10185711.676705401650527No Hit
TAAGCCATTTCGTCTATGCTTCTTCATCAGGGGGCCAGAATTGGACTCTT9994341.6452033156188353No Hit
AATGCCAAGAGCTCAGCTTCCCTCCCAGTTGGTGATGGTGAAGTAGGCAG9178681.5109346659213407No Hit
GACAACTCATTAGTAGCCTGACTGTTAATGGCATTTATGTGAGATGTGGT9124461.5020093217992823No Hit
TTTAACGGTCACTTATGTTCTTAATGTTATACCTAAGAAACCATTACCTA8881381.4619950715375716No Hit
ATTTCCTTACTGCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAAT8659001.425388320783913No Hit
GGACACAGTCAGCCAGAAGGCCGAAGGGCCGGCCTCAACGGCACCTCTCC8545491.4067030420805777No Hit
TGACACGCCAAAGGACCCTGCGGTGATCTCCAAGTCCCCATCCATGGCCC8484971.3967406328908512No Hit
GCTGATGGCTTTCTCTTCCGACCTCTCAGAGGGCACTGGCCGGCTCTTCT8070871.3285741813795198No Hit
TGGGTGGTAGACTGAGCTTAGACAGAGGGTCTTGGCTTGGATACCTATAC7958911.310144053605531No Hit
CGCCTATTGGTAGGGGTGCATCAGGAAGATGCTGGGCTTAATACCTAGGT7843141.2910867483858577No Hit
AAGTGAGGTAACCAACAAAAGGGGGTGATATCATATATATCTGTTGTAAG7617491.253941711478029No Hit
AGAATGCCAGACATCTGTGGTTGGGCCAGAAGAGGAAGGTTTCTTCCTCA7580411.247837842794039No Hit
TAGCATCACTTAATTTGTGTAAGCCTCCTTGGACACAAGCAGTTCTATCA7384931.2156591952658211No Hit
TTTTAACTGGCATGAGTCTTTGATCTGGGTAACCCAATTCTCAGGGTCAG7195941.184548889370804No Hit
TTTTGTTTCCCCAGGCTGAGAAGAAAGCCAAGGTCATTGCAGGAATGAAT7071301.1640314623812547No Hit
CTCATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCA6838911.1257769304645238No Hit
TCTTTAACTGCATTTTCTTGGGCTACAGAACTCACAGATTCTTTCTTATC6298171.0367638249580342No Hit
GGGTGAGGAAAAGTCTGGAGTATTTCCTCTTTATTCTTTATTTGTAATTT6233411.0261034545164167No Hit
ACTCCAGTAATTCTGCCGACCAACGAACTGGTCCCTTTGTTCTTCCCTCT6120281.007480728944147No Hit
TACGAGGTGCGGCAGAAGTGCCGGAACATTGAGGGTAAGTTTGTTGCCTG6105641.0050707872630895No Hit
TTTATATCCCTGTAGAACTGAAGCTTGTTGTGACTTCTGCTCCTGTTCTT6090021.0024995243492838No Hit
TGGTAAATGGTCTGTTTTGGAGAAGTGCACCTGGTGTGAGGGTTTTTGGA6066200.9985784307124811No Hit
AGTTCATAGGGCTGGTGCTTCCATAGATATCTGAAGTGTGTGAAGAGTTT5740510.944965458984093No Hit
GGTGTGGCTATCAAGTTCTGCAGCAGTGGTTTGTCTAGTCAAAACTGTGA5631270.9269830799377327No Hit
GTTCATTCTAAACACACAATATGCTTCTAATATAAAGTAGAAAGAGGTCA5558190.9149531251527818No Hit
TTCCTCCAAATTCTCGATTGTCTTCTCTAGTGAGAGTGCATACCTGGTAG5476420.9014926790284603No Hit
GTGCCGTGACTCACATTGAGCCCATCCCTTGGCAGGCGGGGACTGTGAAC5384070.8862906220444675No Hit
AAAGTTCAACATCGGCACCAAATTTAAAAAGCCCTCTTATATCCTAAGTT5366210.8833506248843795No Hit
GCCTTGAGCCTGACCCATCTGCCTTCCAGCCTGTCCTGACAACCCCAACC5307620.8737059197550656No Hit
AGTGGGGAGAGTTTGAGTAGAATCTTCGCCTTCACAGGATTGAAGAAATT5261060.8660415150644896No Hit
ATTGGGTGGTAATCTAGAGGTGGCTCCCATGAAGTGGCCATCCATCTCAT5220790.8594125293065534No Hit
GGACTTCTTTGCCAAGTTCACAGATAATTTCTCTTTCTTCCTGTCTGCCT5128590.8442351643479812No Hit
CCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCATCT5122970.8433100364622201No Hit
CTCTGAAACTAGGTGTGTATTGTTTGAACAAGAAACCTGTATTTTGCATG5119350.842714135582068No Hit
CCCACCCTAATGCCCTAATGTCTGTCTCTCTGTCCTAGGGCTCCCGGGGC5102570.8399519210050089No Hit
GATCTTTTGAGGGTATGACTACACCTTGGACATTCTCTCGAGCTATCTCA4891940.8052793789093031No Hit
TGCATCTGACCTGTTGTGCTCACTGCTTAGGACCCTCCAAAGGTTTACCC4858670.7998026876709984No Hit
GGCGTGGGTTGTATTAGGTGGACAGCTTGTCCCAGCTGAGCATGGTCAGG4837940.7963902497578618No Hit
TTTCACACTCTTCCTCTTTCTCACGGGCTTTTTCAGCCATTTTGGCTTCC4832160.7954387837116519No Hit
AGGAGACCAAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCC4791460.78873901414751No Hit
ACAAATTGCTGCCAGACTCAAGATTTAAAAAAAGAAGGAAAATCCCATAT4773650.7858072476625624No Hit
GCCACCATCTTGATTTGAATTCCCGTTGTCCCAGCCTTAGGCCCTTCAAA4690210.7720718969885573No Hit
GGATTCCGCTTGGTGAAAACGAGGGGCCTTCAATTCAATCCATCCTGGTT4622600.760942378063947No Hit
CCTGCTGTGTGGTTAGACGGCTTCCGGGCAGCCTGGTCTGGCCAGCACTC4539400.7472465346306151No Hit
GTGAATGAGGTCCTGTGACTCCCTCTGTTATACATGAAGTCCGACCTCTG4522130.7444036594371819No Hit
ACCAGGGCTGAGAGTCTCCTCTGCTCACTGGGTCTCCTTCCAGGTGCTTT4469790.7357877887003957No Hit
TGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCAACTTATACCATAA4373480.7199338622452972No Hit
ACTGTGTAATGATTTCTGCTCCTTGGGGCTCCAGGACGGCCGGGGCTTTG4372230.7197280953667917No Hit
CCACCGAGTCCATGTCCCTACAGTGTGTTCGAGGGCGAGCTCTCCGATAC4194920.690540475184531No Hit
TTACTGCTTTGTGTGTGAAGGCTGGAAAAATTCTGACATATACAAAAGGA4118580.6779738803804376No Hit
ACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAA4117510.6777977439324367No Hit
CACGAGTGCAAATGTTTTGGCTTCTTCTTGTGTAGCTCATCTCATATCTC3947490.6498101561856207No Hit
AGCAGAGAAGAGGGGAGAGATTTATTTTATTTTAAGGAATTGGCTTACAT3884990.6395218122603413No Hit
TATCCTCTCTTCTCCTTTTGTTTCGTTAGGTGGTATGGTCAGGAAAAAGA3842570.6325389074713755No Hit
TGGAATGCTACACTGCTGGGATCCACCTCTGGCCTCACAGGCTGTGTCTG3812870.6276498864380828No Hit
GGTAGACGCATGACCAGTGTTGGCTGGTGAATGAATTCGTGCTTATTCCT3687210.606964553675673No Hit
AACAGAATTCTTCACCAGACGCTAGCTTTGTGGTTCCCTGGATGTTATTT3686360.6068246321982892No Hit
AGGAAAAGAAATGATATTAGGCATCCTCTAGGAGCTTCAAAAGATAATTT3656390.6018911655192393No Hit
GGAAGAGCTATTTGAATATGCGAGTAGAATGTAAACGAAGAAAGGGGTGC3565580.5869426133295652No Hit
CTCCTCCTTGGCTCATCTTCAAACCGTCTCCTGTTTTGTAGTCCAACCCT3550040.5843845194959838No Hit
GGCCCTGTAGAAGTTAGAAGGACTTTTCCTTGAAATAAACGAAGACCCTC3532490.5814955525217652No Hit
AATCTGGTATGGTGGAAATGGGTGCAAAGCCTTGAAGTCAGAGGGCCGTG3450330.5679709071313499No Hit
AGTACTTCAAACCTTCCCATCTCAGAAATGTACTAATTCTAACACAGTCT3381480.5566372674632621No Hit
CCGGGTTTGAAAAGAGTGAAGTATGCCTCCCCGGCCCGCAGCAGCCGCGG3366240.5541285576805219No Hit
TAACGGTGCTGTCTGCTGGCTGGTGCAGGGCTCCTGGTGCTGAAGGACTT3141570.5171448420054415No Hit
GTTTTCATCTCATGGGCTAATTCTGCCTAAGGAGTTATATTGAGGAGTCT3052740.5025222245513203No Hit
TCGGAGAAGGGCTGCATACATGTGTAGATGGATTAGGACTCTGGGAAGGT2972160.48925766849533614No Hit
CAATTGCTGCCAATGATTATTTAAACTTACCTGATTATTATATGCATCAG2890680.4758449602868278No Hit
AAACGAACACACAGAATCACACTAGCCTCATCTGGTAGATCACACTAGAC2869130.47229753930139146No Hit
GCTGTCTTTGTGAACTCTAAATAATTGAGATACTGAAATACTTAAGAAAT2799540.4608420856412283No Hit
GTTTGAAGAGAGTTATGACGTGTGTGCGTGATTTGGGGAAGGGGCTCTTA2747570.45228712190048No Hit
CAAGAGAAGAATGTGAGCCTGTGTCCGGTGGGACTCCAGACACACAGGCA2616070.43064044628169207No Hit
GGCAGTGTCAAACAGTTGTAGTCCTGGCGGGCAGGTGGGAGCACCTCTGC2516450.41424164913231065No Hit
TGGCACAGAAATAGAAAGTGATGACCTCAAGGTTACTTACAAATTAGTAG2515270.41404740519900146No Hit
AGTCAAACAATTGTAACTTGAACCATCTTTTAACTCAGGTACTGTGTATA2482580.4086661897923232No Hit
CTCTTGCAGCAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGC2265820.37298456692442616No Hit
GATACTTGGGAAAGGATCCGCCGGCCTTGAACTCCCGGCTCCGCCGCCCC2187080.36002289971360213No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2140620.3523749563733064No Hit
ACTTCCATCCTGACATGCTGATAGTCTCTCCTATAAATGTGAATTAAAAT2084480.34313355432586345No Hit
ACTTCCATCCTGACATGCTGATAGTCTCTCCTATAAATGTGAATTAAAAA2076270.34178207746783873No Hit
ACGTTATAAATGGTTATTAGGGTTAGCTATGCTGCAGCTGTTACCCGCCT2063590.33969477825227806No Hit
AATCTTCAGCATGTTCCAGAGTAGTACTCAGTTAGCCAGCTGCACTGACC2034520.33490946372575203No Hit
CTATTGCACCCAGTTCTCCAAGGGCAATGGATTCCCCCTTGTTTCTTCTC2001050.3293998497868864No Hit
CCAGGTGGGAGTTTAGCTTTGGCCTTTAACCCTTCTCATGGGAGAAAGTA1976680.32538821872354146No Hit
AAGGGGCTGGAGTTTCCTGTCAGCCTGTAACTGACCTTGGCACCTGCTTT1961120.3228268326199039No Hit
CTGGTCTCTGCTTCTGTCATTACATGTGTATCTCTTTCTGTGTCTCTTCC1948380.3207296565941749No Hit
GAGACTGACTCTCGAGGCTCCCAGCCCCTGCCAGTTGCAAGGCATGGGGT1880150.3094980772978259No Hit
CTGGTCTCTGCTTCTGTCATTACATGTGTATCTCTTTCTGTGTCCCTTCC1876330.3088692537171128No Hit
TACCCAAAACCATTTTCCCATCTTCCTTGCTAACAGAACTTGATTTGGGC1864830.3069761984348614No Hit
CCCTCTAATTTGGGTTTCTCCAGGGAACAGGCACGGTCTCCTGGTACCCA1856440.3055950911463319No Hit
AAAGCGATTATACATCAGGAAGTAAACAAACCTCTTTTGGGTCATCCCCA1850770.30466173258543056No Hit
GATACTTGGGAAAGGATCCGCCGGCCTTGAACTCCCGCCTCCGCCGCCCC1787710.29428120509858063No Hit
CTTTCTTGTGTAAAGTCAGGACTCACACGACTATTCTGGCTTCCCTTCAT1781270.29322109414051983No Hit
ACACACCTAAAGTCCCACCTACGTGGGAGGCTGAGGCAGGACAACCACTT1664650.2740238674434624No Hit
CCTCTCATTTCCAACAGCAGACCCTACTAGGGGATTAGAGGTCCCTTTCC1625070.26750846500246145No Hit
TTTAACTCTCGCCTCCAAAGACCACGATAATTCCTTCCCCAAAGCCCAGC1614530.2657734386829023No Hit
TTTCACATGGGTAATGGACACTATATTACCAGAAATATTAAAGACAAAAT1569790.25840863056743035No Hit
CAGACCAGGGACTTCTTTAATGGAGAACCTGCCTGGTTTTCCTTCCAGTC1500930.24707334476431445No Hit
ATGGATGATTTGATGCTGTCCCCGGACGATATTGAACAATGGTTCACTGA1455920.23966409100308522No Hit
AAGTGAGGTAACCAACAAAAGGGGATGATATCATATATATCTGTTGTAAG1428000.23506808200478438No Hit
TGGAACAAACACAGAAGCTACTTGAAGCATTCAATTGCAGTTTACACTTG1231840.20277749729465938No Hit
GAGCATCTTAGCAGGGCTTCAGTCAATTTCCCCAAGTTTTGCACAATGAG1217890.200481138930537No Hit
GCAAGACAAGAGGAAGAGAAGATGACAGCCAGAAGAAGTGAGTGCGGTAC1101640.18134481922951728No Hit
AAATTTAGCAGTAAGTGTATAGTGTGTATACAGTCTACAGTGGCATACAG1039830.17117005862117293No Hit
CTGTCCTCAGCCCAACTTACCAATGTGCTGCCATTCTGCATGTTGTGCAA989680.16291469145552873No Hit
TAGTGCTATTTTAGAGCAATATACCAAAACATTTGTGAACGACATGATAT889520.1464270030146329No Hit
TAGTGCTATTTTAGAGCAATATACCAAAACATTTGTGAATGACATGATAT884090.14553315169440464No Hit
ACGTTATAAATGGTTATTAGGGTTAGCTATGCTGCAGCTGTTACCCACCT690960.1137413458977772No Hit
ATTGGGTGGTAATCTAGAGGTGGCCCCCATGAAGTGGCCATCCATCTCAT651230.10720125143135555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)