FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004939420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004939420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42807
Sequences flagged as poor quality0
Sequence length250
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA33887.914593407620249No Hit
NCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA33187.751068750438013No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA30787.1904127829560585No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA30297.075945522928492No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG29646.92410119840213No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG28506.657789613848203No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA14073.286845609362955No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA12472.913074964374985No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA12012.8056159039409443No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA10752.5112715210129184No Hit
NCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA6911.6142219730417924No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA6001.4016399187048847No Hit
NCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA5801.3549185880813885No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA5211.217090662742075No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA4771.1143037353703833No Hit
NCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA4331.0115168079986918No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA4301.0045086084051673No Hit
NCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTGCGTCCCAGGG4230.9881561426869437No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA3870.9040577475646506No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTGCGTCCCAGGG3500.8176232859111827No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA3330.7779101548812111No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA2670.6237297638236737No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA2550.595696965449576No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA2540.5933608989184012No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA2330.5443035017637302No Hit
NCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA2270.5302871025766814No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2190.5115985703272828No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA2120.4952461046090592No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA2070.4835657719531853No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1870.4368444413296891No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1860.4345083747985143No Hit
NCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1830.42750017520498984No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1770.41348377601794095No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1760.4111477094867662No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1680.3924591772373677No Hit
NCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1540.3597542458009204No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1540.3597542458009204No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1490.3480739131450464No Hit
NCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGTCATAGTTTGGTA1370.32004111477094865No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1340.3130329151774242No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGTCATAGTTTGGTA1260.2943443829280258No Hit
NCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA1260.2943443829280258No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTGCAGTCCAGTG1230.2873361833345014No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTGCAGTCCAGTG1170.2733197841474525No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAATCCAGTA1150.2686476510851029No Hit
NCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA1120.26163945149157847No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA1060.2476230523045296No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA1020.23827878617983042No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA1000.2336066531174808No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA950.22192632046160676No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCAGTG950.22192632046160676No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT900.2102459878057327No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA900.2102459878057327No Hit
NCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA890.2079099212745579No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA850.19856565514985866No Hit
NCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA750.1752049898381106No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA680.15885252411988693No Hit
NCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT680.15885252411988693No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG660.1541803910575373No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCCCAGCA660.1541803910575373No Hit
NCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA650.1518443245263625No Hit
NCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCCCAGCA620.1448361249328381No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGCA620.1448361249328381No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGCTAGTA600.14016399187048847No Hit
NCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAATCCAGTA570.13315579227696406No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA560.13081972574578923No Hit
NCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA550.12848365921461444No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA550.12848365921461444No Hit
NCTGTTTGATACCCACACTTTCGCGCATCAGTGTCAGTTGCAGTCCAGTG520.12147545962109No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG500.1168033265587404No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA480.11213119349639078No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGCA470.10979512696521598No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTCAGCA450.10512299390286635No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA440.10278692737169155No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGCA430.10045086084051674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)