FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004939424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004939424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43792
Sequences flagged as poor quality0
Sequence length250
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA544112.424643770551699No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA471310.762239678480087No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA30757.021830471318963No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA26636.081019364267446No Hit
NCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA23075.2680854950675915No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA22195.0671355498721224No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA13663.119291194738765No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA12132.7699123127511873No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA11622.653452685421995No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA10522.402265253927658No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG9302.1236755571793933No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG7941.8131165509682134No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG4431.0116002922908294No Hit
NCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA4210.961362805991962No Hit
NCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG3530.8060833028863719No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA3260.7444282060650347No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG2480.5663134819145049No Hit
NCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA2360.5389112166605773No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG2340.5343441724515894No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA2250.5137924735111435No Hit
NCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA2220.5069419071976616No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA2180.49780781877968583No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2140.4886737303617099No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG2060.4704055535257582No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1930.44071976616733655No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1880.4293021556448667No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1830.41788454512239676No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG1780.4064669345999269No Hit
NCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1770.40418341249543294No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1740.397332846181951No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1640.37449762513701135No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1270.29000730727073437No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1240.28315674095725246No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA1240.28315674095725246No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1220.2785896967482645No Hit
NCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1190.2717391304347826No Hit
NCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1180.26945560833028864No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1180.26945560833028864No Hit
NCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT1130.2580379978078188No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1090.24890390938984291No Hit
NCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1080.24662038728534894No Hit
NCTGTTTGCTACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1080.24662038728534894No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT1070.24433686518085496No Hit
NCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCCCAGCA930.21236755571793936No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA920.21008403361344538No Hit
NCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA880.20094994519546952No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAGTCCAGCA860.19638290098648153No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA840.1918158567774936No Hit
NCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG840.1918158567774936No Hit
NCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA830.18953233467299963No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCCCAGCA810.1849652904640117No Hit
NCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG770.1758312020460358No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG750.17126415783704788No Hit
NCTGTTTGCTACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA730.16669711362805992No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA730.16669711362805992No Hit
NCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA710.16213006941907196No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG700.159846547314578No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA630.1438618925831202No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA600.1370113262696383No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA590.13472780416514432No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA590.13472780416514432No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTCAGCA590.13472780416514432No Hit
NCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTCAGCA580.13244428206065034No Hit
CCTGTTTGCTACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA570.1301607599561564No Hit
NCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAGTCCAGCA550.12559371574716843No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA530.1210266715381805No Hit
NCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTCCGGTA500.11417610522469858No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA490.1118925831202046No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA460.10504201680672269No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA460.10504201680672269No Hit
NCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA450.10275849470222873No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA440.10047497259773476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)