FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004939426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004939426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26085
Sequences flagged as poor quality0
Sequence length250
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA396215.1888058271037No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA370414.199731646540156No Hit
NCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA9643.6956105041211424No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA9393.5997699827487057No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA7312.8023768449300364No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA6952.6643664941537284No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG5312.0356526739505463No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG5292.0279854322407513No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG4851.8593061146252636No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA4791.836304389495879No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA4591.75963197239793No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG4301.6484569676059035No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA3631.3916043703277747No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG3421.3110983323749281No Hit
NCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTGCCCCAGGG3301.2650948821161587No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTGCCCCAGGG3251.2459267778416714No Hit
NCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA3191.2229250527122866No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG3151.207590569292697No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2971.1385853939045427No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA2901.1117500479202607No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2761.0580793559516963No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA2661.0197431474027219No Hit
NCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG2090.8012267586735672No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2050.7858922752539774No Hit
NCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2010.7705577918343876No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1880.7207207207207207No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1850.7092198581560284No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1830.7015526164462335No Hit
NCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1590.6095457159286947No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1360.5213724362660533No Hit
NCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA1290.4945370902817711No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA1280.49070346942687365No Hit
NCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA1250.47920260686218136No Hit
CCTGTTCGCTACCCACGCTCTCGTGCATCAGCGTCAGTTACAGTCTGGTA1240.4753689860072839No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA1170.4485336400230017No Hit
NCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA1150.4408663983132068No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA1120.42936553574851444No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA1090.41786467318382214No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1060.40636381061912974No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1030.3948629480544374No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA1000.3833620854897451No Hit
NCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1000.3833620854897451No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA980.37569484377995016No Hit
NCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA810.3105232892466935No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCCCAGCA800.30668966839179607No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA740.28368794326241137No Hit
NCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTGCAGCCCAGCA650.24918535556833432No Hit
NCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG650.24918535556833432No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA650.24918535556833432No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG600.23001725129384704No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA600.23001725129384704No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT590.2261836304389496No Hit
NCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA540.20701552616446234No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA480.18401380103507764No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG470.18018018018018017No Hit
NCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT470.18018018018018017No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA470.18018018018018017No Hit
NCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA460.17634655932528273No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT450.17251293847038526No Hit
NCTGTTTGCTACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA410.15717845505079547No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCCAGCA410.15717845505079547No Hit
NCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTCAGCA390.14951121334100057No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG390.14951121334100057No Hit
NCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT380.14567759248610312No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTTGTATCCCAGCA370.14184397163120568No Hit
NCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCAGTTGTATCCCAGCA360.13801035077630822No Hit
CCTGTTCGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCC360.13801035077630822No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA340.13034310906651334No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCACTGTCAGTTGCAGCTTAGCA330.12650948821161587No Hit
NCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA330.12650948821161587No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCTAGCA330.12650948821161587No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA320.12267586735671841No Hit
NCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA310.11884224650182097No Hit
NCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCCCAGCA310.11884224650182097No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTCAGCA290.11117500479202608No Hit
NCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT270.10350776308223117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)