FastQCFastQC Report
Fri 24 Mar 2023
EGAF00004939464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004939464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686613546
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCCCGA21874853431.859047243440198No Hit
GTTTATCT15845592523.07789089264487No Hit
CCAAGATG15270587022.24043945675374No Hit
TACGTGAC13474433119.624478978747092RNA PCR Primer, Index 17 (100% over 8bp)
NGGCCCGA23147500.3371255946645713No Hit
NTTTATCT16846130.2453509706899957No Hit
NCAAGATG16135380.23499944173836618No Hit
NACGTGAC14249210.20752882146021626No Hit
AGACCCGA6990070.1018050115777937No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)