FastQCFastQC Report
Mon 13 May 2024
EGAF00004939571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004939571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences641048869
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGGTAT19350322830.185409780357947No Hit
GGTTCCTC16460680125.677730507001336ABI Solid3 Adapter A (100% over 8bp)
ATCATGCA13455619620.99000598969936No Hit
TAACAAGG12793861519.957700759940035No Hit
NCGGGTAT20517030.3200540706359159No Hit
NGTTCCTC17479180.2726653277973415No Hit
NTCATGCA14314100.22329186887622446No Hit
NAACAAGG13627720.21258472885629567No Hit
ATCATCCA9702650.15135585552370734No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)