FastQCFastQC Report
Wed 15 May 2024
EGAF00004939638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004939638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711146807
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCATTC19382682127.25552854798939ABI Solid3 Adapter A (100% over 8bp)
AGCATCCG18494156926.006102703348No Hit
TAATGGGA15665898922.02906452760042No Hit
GTGGCAAT11630607316.35472055209551No Hit
AACATCCG272492023.8317266887482484No Hit
AGCATCCA83923241.1801113240462036No Hit
NCTCATTC20344870.2860853736491571No Hit
NGCATCCG20037910.2817689653213897No Hit
NAATGGGA16375110.23026342576266393No Hit
NTGGCAAT12208030.17166680465739614No Hit
AGCAACCG10856550.15266257111943976No Hit
CCTAATTC10401220.14625981439581995No Hit
ATCATCCG10365990.14576441738843382No Hit
AGAATCCG9686540.13621013136321372No Hit
AGCATACG9346330.13142616838044807No Hit
TAATGGAA7798040.10965443313872601No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)