FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004942993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004942993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64928588
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA3165400.4875202276075987No Hit
CCCATGTCTGCCTGCTGTGACCTGCAGCTCCTCGTACTTGGTCTGGTACC1624110.25013788995380587No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC1342590.20677948517839323No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1039700.16012977211209337No Hit
CTCATATTGGGCCTTGACCTCAGCGATGATGCTGTCCAGGTCCAGGTTGC990860.15260766182070676No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA975640.1502635480075433No Hit
GTCGATCTCAGATCTCAGCCTCTGGATCATGCGGTTGATCTCAGCAATCT944600.14548291116387746No Hit
CCAGGTTCTGCCTCACAGTCTTGGTGCCCTGCTCCTGCAGCAGGGTCCAC940330.14482526556714895No Hit
GCGGTTGTTGTCCATGGATAGCACCACGGATGTGTCTGAGATGTGGGTCT880660.13563516890279517No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT791460.12189699859174513No Hit
GTCCAGGTTGCGGTTGTTGTCCATGGACAGCACCACAGATGTGTCTGAGA756350.11648951922379708No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC722090.11121295291374579No Hit
GTCCAGGTTGCGGTTGTTGTCCATGGATAGCACCACGGATGTGTCTGAGA705370.10863781605723506No Hit
CTCATATTTGTTCTTGAAGTCCTCCACCAGGTCCTGCATGCCTCTGAGCT654410.1007891931979177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)