FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004943007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004943007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8319306
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT6574947.903231351268963No Hit
GTACCGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC3524124.2360744994834905No Hit
TGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCAT2209982.656447545023587No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC1944852.337755096398666No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG1779492.138988516590206No Hit
ACCGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTG1606551.9311105998505163No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT1477201.775628880582106No Hit
CGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTG1016431.2217725853574806No Hit
TTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCA954701.1475716844650263No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG947481.1388930759368632No Hit
CCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTG754730.9072030767951076No Hit
GCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATG729190.8765034006442364No Hit
CCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACA716530.8612857851364043No Hit
GCCCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACA658640.7917006538766576No Hit
TTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTC611990.7356262649793144No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT596600.7171271257482295No Hit
GCCGGCTTCACGCTCAGAAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC579400.6964523242683945No Hit
GCCGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTG528750.6355698420036479No Hit
GTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGG519550.6245112272586198No Hit
CCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA450390.5413792929362137No Hit
CTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT381760.45888443098498843No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG316040.3798874569585492No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA282450.3395114929057785No Hit
AATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGC257760.30983353659548046No Hit
CCCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACAC226560.27233040832973326No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT223130.2682074682671848No Hit
TGGGTTTGTTAGGTACCGTTTGCATTAATAAATTAAAGCTCCATAGGGTC217620.2615843196535865No Hit
GTGGGTTTGTTAGGTACCGTTTGCATTAATAAATTAAAGCTCCATAGGGT213030.256067032514491No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC208390.25048964420830294No Hit
TACCGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCT206290.2479653951904161No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC192200.23102888630373736No Hit
GCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCT191060.22965857969402736No Hit
GTTTGTTAGGTACCGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC186150.22375664508553958No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT177370.21320288014408895No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT164960.19828577047171964No Hit
CAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAG162600.19544899538495156No Hit
CATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGC159950.1922636335290468No Hit
GCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTA153840.18491927091033794No Hit
GGGTTTGTTAGGTACCGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT152180.18292391216286552No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT151810.1824791635263807No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCG148350.17832016276357668No Hit
TAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCG145480.17487035577246468No Hit
ATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTG144080.17318752309387347No Hit
GAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAG138820.16686488031573787No Hit
CGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTC137690.1655065939394464No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT137440.16520608810398366No Hit
CAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGT135530.1629102235210485No Hit
GGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC131200.15770546245083425No Hit
CGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGG127210.15290938931684928No Hit
CTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA126520.15207999321097215No Hit
CGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGT125670.15105827337039893No Hit
TTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC124760.14996443212931462No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAG123840.14885857065481184No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG123680.1486662469201157No Hit
GTTTCCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAA117830.1416344103702881No Hit
CCGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGT117720.1415021878026845No Hit
GTTAGGTACCGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT116840.14044440726185572No Hit
TCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCT115830.13923036368658637No Hit
GCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAG112110.13475883685490112No Hit
GGTTTGTTAGGTACCGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT110660.13301590300921737No Hit
GAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGT109770.13194610223497008No Hit
GCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCC109600.1317417582668554No Hit
TGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTA106610.12814770847472132No Hit
GGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAA105670.12701780653338152No Hit
GTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGC103090.12391658631140626No Hit
CCTGAACATGCCCTGTTCCTACTCTCTCACTCCAGTTACCACAATTTCTC94300.11335080113653712No Hit
CCGGCTTCACGCTCAGAAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG93880.11284595133295974No Hit
CTCGGGGAAGCACAGCTCTGTCTTGCACCAGTGCCAGACGCCACCAGAAC90560.10885523383801486No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTG90310.10855472800255213No Hit
GGTACCGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTG89910.10807391866581179No Hit
CGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGG84510.10158299261981708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)