FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004943008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004943008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8319306
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT88457010.632737875010246No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC1335031.604737222071168No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT1147071.3788049147368782No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG991401.1916859411109533No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT958271.1518629077954339No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG906311.0894057749528627No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA891041.0710508785227997No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCC766950.9218918020325253No Hit
GTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGA766000.9207498798577669No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT686520.8252130646474598No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA674140.810332015675346No Hit
TGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG665260.7996580483997102No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG608210.731082616747118No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA594690.7148312611652943No Hit
ACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCG587510.706200733570805No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC587380.7060444705363644No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG546100.6564249469847605No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC499110.599941870151188No Hit
CAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGC469580.564446120866332No Hit
GGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA459180.5519450781110828No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT436790.5250317754870418No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA396500.4766022550438702No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTG382090.4592810986877992No Hit
GGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAA376680.4527781524083859No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC359320.4319110271938549No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG317470.3816063503373959No Hit
TTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAG308210.3704756141918569No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG296840.35680860879501247No Hit
GGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAG289200.3476251504632718No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT277290.3333090524618279No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGAT267370.3213849809106673No Hit
TGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGAC267250.3212407381096452No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA249830.30030149149460306No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT241940.2908175273273997No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA238840.28709125496766197No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGAC236690.2845069047826826No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA236490.2842665001143124No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTA228800.2750229406154792No Hit
CTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAGGGCTCTGCCC226800.27261889393177746No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA213820.2570166309545532No Hit
GTCCAAGTGGCATAGGGGAGCTTAGGGCTCTGCCCCATGATGTACAGTCC210470.25298985275935276No Hit
GGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAGG206570.24830196172613436No Hit
GGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAGGGCTC203010.24402275862914524No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC202320.24319336252326818No Hit
GGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAA197870.2378443586520318No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG190410.2288772645218243No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA189210.22743483651160326No Hit
GTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACG189040.2272304925434886No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA189030.22721847231007009No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA170720.20520942492078068No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG168770.20286547940417146No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGAT166580.20023304828551805No Hit
GGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG162800.19568940005332175No Hit
GTTTATCCATTCGCTGAGTGCGCTGCACTGACCTTCTTCCAAGCCTCAGT161170.19373010200610483No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA152700.18354896430062795No Hit
GTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAG145940.17542328650971606No Hit
GCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAAC140770.16920882583234706No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTA139210.16733366941905972No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTT138870.16692498148283041No Hit
CGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG135100.16239335348405265No Hit
ATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACC132010.15867910135773344No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATA131450.15800596828629696No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG128520.1544840398946739No Hit
TGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC123450.14838978155149No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT121850.1464665442045286No Hit
GGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAA120720.14510825782823714No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG117420.14114158080012926No Hit
GGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAGGG116140.13960299092256012No Hit
CTGCACTGACCTTCTTCCAAGCCTCAGTTCCTGTTCTAGGAACTTGAGGC115960.13938662672102697No Hit
CTGACCTTCTTCCAAGCCTCAGTTCCTGTTCTAGGAACTTGAGGCTATGT113530.13646571000032937No Hit
AGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTCAGTAA106370.12785922287267712No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAG101290.12175294429607471No Hit
CTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG97900.11767808516720026No Hit
CTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGT93050.11184827195922353No Hit
TGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGG91300.1097447311109845No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC90710.10903553733929247No Hit
CGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGG88180.1059944182844098No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAA86420.10387885720275225No Hit
AGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG84610.10170319495400218No Hit
ATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC83750.10066945488001042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)