FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004943014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004943014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18322707
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA6383163.483742877075969No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATC635830.3470175012895202No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCA467040.25489683374841937No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA419280.2288308163198811No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC365750.199615700889612No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT359580.1962482945342083No Hit
AGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA295540.16129712711118505No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT293730.16030928181081538No Hit
GACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCAT271580.14822045672618134No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCG266230.145300582495807No Hit
ACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATC265780.145054985597925No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCG252000.13753426281389536No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC239930.13094680824181712No Hit
GAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAG198440.10830277425710076No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT197230.10764239148724039No Hit
GAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAG197020.10752777960156214No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT192630.10513184542000262No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)