FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004943025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004943025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2562712
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGATCATCAATGGCTGACGGCAGTTACAGCCAAGCAACGCCAGAAAG189280.7385925535136215No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT180500.7043319733157686No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA174750.6818948051907511No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCC173140.6756123981157461No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT145200.5665872716091391No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG138850.5418088337667284No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTC124750.4867889954079897No Hit
GAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAG121160.47278039826558743No Hit
GCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAG117820.45974733017209896No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT111960.43688092926555927No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT107240.4184629408220666No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT100400.39177246604378485No Hit
CCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTG99180.3870118842850855No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCG96860.37795897471116535No Hit
GAAGAACGATCATCAATGGCTGACGGCAGTTACAGCCAAGCAACGCCAGA93810.3660575203144169No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT93620.3653161182372424No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT91950.3587995841904982No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGC89860.3506441613415788No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTACAGCCAAGCAACGCCAG87610.3418643999013545No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGT86940.3392499820502655No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC85320.33292855381330405No Hit
GTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGG82540.32208067078938246No Hit
AGAACGATCATCAATGGCTGACGGCAGTTACAGCCAAGCAACGCCAGAAA79530.31033530104046025No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG77700.3031944284024112No Hit
GGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAAT74460.29055157192848824No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC69560.27143120256977765No Hit
CGATCATCAATGGCTGACGGCAGTTACAGCCAAGCAACGCCAGAAAGCCG68060.2655780282762948No Hit
GTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATT67640.2639391394741196No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT66280.25863226144802853No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66250.2585151979621588No Hit
GTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATA64920.2533253834219374No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC60020.23420501406322675No Hit
GGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTG55480.21648940653495202No Hit
TGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC53330.2080998567142933No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA53220.20767062393277125No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACTTCTCTTCCTCGTGGTTTTC51980.2028319998501587No Hit
CTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAG51800.20212961893494077No Hit
GCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGGCTCT51730.20185647080124494No Hit
GGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG51680.20166136499146217No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA50990.19896890481646007No Hit
GTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATA50930.19873477784472074No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCG50870.19850065087298144No Hit
GTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCA50720.19791533344363316No Hit
GTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTA50200.19588623302189243No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT48790.19038424918601857No Hit
GCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA48530.1893696989751482No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTT47740.18628702718058057No Hit
GTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG47600.18574073091318885No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG46750.18242393214688188No Hit
CATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGG46430.18117525496427223No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT44220.17255157817187416No Hit
GGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCT43800.17091268936969897No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT43590.17009324496861136No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTC41180.16068914493708228No Hit
CTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTC40960.1598306793740381No Hit
GGGAGAAGAACGATCATCAATGGCTGACGGCAGTTACAGCCAAGCAACGC40740.15897221381099397No Hit
GCCGGCTTCACGCTCAGGACAAAACGCTACCTCTCTTCCTCGTGGTTTTC40430.15776255779034085No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT40060.15631877479794842No Hit
TGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTA39510.15417261089033804No Hit
GGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAA39090.15253372208816285No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC38150.14886573286424692No Hit
TGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTAT37590.14668054779468No Hit
CTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGT37590.14668054779468No Hit
AAGAACGATCATCAATGGCTGACGGCAGTTACAGCCAAGCAACGCCAGAA37470.14621229385120138No Hit
CCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGAC37230.14527578596424412No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT36640.14297353740880756No Hit
CGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCA35780.13961771748054405No Hit
GAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGA35650.13911044237510886No Hit
CCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT34610.13505224153162743No Hit
CAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCAC34290.13380356434901777No Hit
TTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCA33960.13251586600445153No Hit
CAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTA33280.129862426991406No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT32990.12873081329466596No Hit
GTTTCCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAA32880.1283015805131439No Hit
CTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTA32860.12822353818923077No Hit
CATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTT32290.1259993319577073No Hit
AGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAA31940.12463359128922796No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC31790.1240482738598797No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG31440.12268253319140036No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGG30860.12041930579792033No Hit
GGAGAAGAACGATCATCAATGGCTGACGGCAGTTACAGCCAAGCAACGCC30840.12034126347400723No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG30040.11721957051748304No Hit
GTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC30000.11706348586965683No Hit
AGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAG29950.11686838005987407No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC29650.1156977452011775No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA29440.1148783008000899No Hit
ATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGT29300.11433200453269818No Hit
CCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTG29120.11362962361748023No Hit
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA28640.11175660784356573No Hit
CGGGGAGCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGC28250.11023478252726018No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC28240.11019576136530364No Hit
GGGGATTCGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAA28220.11011771904139052No Hit
GCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCA28150.10984457090769467No Hit
GGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTT28130.10976652858378155No Hit
GCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAA27320.10660581446530082No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT27280.10644972981747461No Hit
ATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTG27170.10602049703595252No Hit
GCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTA27130.10586441238812631No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC27090.10570832774030013No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA26920.10504496798703873No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA26920.10504496798703873No Hit
GCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATG26670.10406943893812493No Hit
GCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTA26490.10336705802290698No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA26270.10250859245986284No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG26150.10204033851638421No Hit
GCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGC26050.10165012689681868No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC26030.10157208457290556No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT25910.10110383062942696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)