FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004943026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004943026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2562712
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT217060.8469933414289237No Hit
GTCCAAGTGGCATAGGGGAGCTTAGGGCTCTGCCCCATGATGTACAGTCC192290.7503379232625438No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT179930.7021077670842452No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC145450.5675628006580529No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA145390.5673286736863136No Hit
GCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT143890.5614754993928307No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA139660.5449695478852091No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC133990.522844549055844No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT132020.5151573801504032No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG108260.42244309934163493No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA100830.3934503760079166No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACCAGGATT100200.3909920428046538No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTG95120.3711692925307253No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAAGTAGCG90140.3517367538763622No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA89290.34841995511005525No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG86550.33772815673395995No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC79710.3110376819556782No Hit
GGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA79050.3084622852665458No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCC78930.30799403132306713No Hit
GTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACG78390.3058868885774133No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT77140.3010092433328443No Hit
GGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC72750.2838789532339178No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA72430.28263027605130814No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG72260.28196691629804677No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA69840.2725237951045611No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAA64980.2535595103936767No Hit
GTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAG64780.25277908715454567No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT61450.23978504022301375No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG61290.2391607016317089No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC59360.23162961737409432No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG56160.2191428455479976No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA52370.20435382516646428No Hit
GTGGCATAGGGGAGCTTAGGGCTCTGCCCCATGATGTACAGTCCCTTTCC51200.19978834921754768No Hit
GGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAA49810.19436440770558688No Hit
TGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGG49760.19416930189580411No Hit
GCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGT49330.19249139193167236No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG49100.1915939052066717No Hit
ACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCG48780.190345228024062No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCT48540.18940872013710475No Hit
TGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTA47600.18574073091318885No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG45610.17797551968383493No Hit
GCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAG44190.17243451468600451No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAG43850.17110779517948174No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA42990.16775197525121824No Hit
GTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGA41990.163849859055563No Hit
GGAGCACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGG41130.1604940391272995No Hit
GGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTAT41080.16029893331751677No Hit
CTCTGTCCAAGTGGCATAGGGGAGCTTAGGGCTCTGCCCCATGATGTACA40750.15901123497295053No Hit
GGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAA39070.15245567976424976No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG38620.1506997274762049No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAAC38450.15003636772294351No Hit
GGCATAGGGGAGCTTAGGGCTCTGCCCCATGATGTACAGTCCCTTTCCAC38310.1494900714555518No Hit
AGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGA37950.1480853096251159No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA37720.14718782290011517No Hit
GCCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGC37650.14691467476641934No Hit
GTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACT37580.14664152663272345No Hit
CGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGG36880.1439100452957648No Hit
GGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG36060.1407103100153275No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG35940.1402420560718489No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT35910.14012499258597924No Hit
CAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGT34480.13454496642619224No Hit
GCATAGGGGAGCTTAGGGCTCTGCCCCATGATGTACAGTCCCTTTCCACA34420.13431083945445293No Hit
AGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG34250.13364747970119156No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT33840.1320476120609729No Hit
GTCCAAGTGGCGTAGGGGAGCATAGGGCTCTGCCCCATGATGTACAAGTC33550.1309159983642329No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG33210.1295892788577101No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG33120.12923808840010115No Hit
TGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGAC32950.12857472864683975No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTT32790.12795039005553493No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA32750.12779430540770872No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA30770.12006811534031137No Hit
ATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACC30090.11741467632726581No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT29970.11694642238378716No Hit
GTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGA29960.11690740122183062No Hit
GCTCTGTCCAAGTGGCATAGGGGAGCTTAGGGCTCTGCCCCATGATGTAC29430.11483927963813335No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA29430.11483927963813335No Hit
CGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCAGACCCCAGAAA28740.11214681946313124No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT28110.10968848625986846No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA28100.1096494650979119No Hit
GTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAAT27950.10906414766856361No Hit
GAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTA26830.10469377752942977No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAAGTAGCGTTTT26760.10442062939573389No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA25820.100752640171818No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC25740.10044047087616556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)