FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004943053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004943053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42223231
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG2270330.5376968901314066No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC1572660.37246320633302554No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA1463820.3466859274696434No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA1415770.3353059362036979No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG1298640.3075652831968259No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA1126340.2667583634232065No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTT840690.19910603241139932No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT832880.19725633976234552No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG819730.19414194048769032No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA711110.1684167656425914No Hit
GTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACG698030.16531894491920812No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT677170.16037853664017326No Hit
TGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTC642560.15218162721843811No Hit
GAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTA600180.14214449860551884No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC599580.14200239673747375No Hit
GTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAG565160.13385048624061952No Hit
CGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG530940.12574594303311368No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC526330.12465412701363379No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA507040.12008555195598365No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA490900.11626301170557032No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATA465790.11031604852788267No Hit
CGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG462410.10951554133789523No Hit
GAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGA442100.10470539310456844No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG429860.10180651499644827No Hit
TGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG426460.10100127107752603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)