FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004943091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004943091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43463794
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA2717810.6253043625229772No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA2598990.5979666662325889No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC1912220.43995699040907477No Hit
GTCCAAGTGGCATAGGGGAGCTTAGGGCTCTGCCCCATGATGTACAGTCC1678530.38619040022138884No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG1356870.31218397547163046No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1290610.29693910292322845No Hit
TTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1190750.2739636581196754No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA1094880.25190621877142155No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATA1072340.2467202932169244No Hit
CGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG1009300.2322162671763077No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTT989860.22774357894297034No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAA900600.2072069456246733No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTA888620.2044506284932236No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT856490.1970582687742354No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT854580.1966188225537789No Hit
TGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG756090.17395858263086744No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCG727980.16749113066383484No Hit
GTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACG668780.15387059859523539No Hit
TGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTC542150.12473600440863493No Hit
GTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAG541900.1246784852698317No Hit
TGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGG541490.12458415388219445No Hit
CTCTGTCCAAGTGGCATAGGGGAGCTTAGGGCTCTGCCCCATGATGTACA516290.11878622469083118No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA511200.1176151350247979No Hit
ATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAACC485090.11160783616819094No Hit
GGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG483240.11118219454104719No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC469270.10796802506472399No Hit
GGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGA439170.10104272075281787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)