FastQCFastQC Report
Fri 27 Jan 2023
EGAF00004945196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004945196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124095240
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG27021592.1774880325788484No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG17543081.4136787196672491No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTC7269450.5857960385910047No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGT3507220.28262324969112435No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT3260220.2627191824601814No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGA2722920.21942179248776988No Hit
CTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTT2537550.2044840720723857No Hit
CGGGGAAATGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT2390170.19260771001369592No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG2222710.17911323593072548No Hit
GGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCTA2144860.17283982850591206No Hit
GGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGTT2136690.17218146320519628No Hit
GTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATTTGGT2097790.16904677407449312No Hit
GTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATTATTCCTT2097130.16899358911751972No Hit
GTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTG2059200.1659370657569138No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGTATGGCT2031930.16373956003469592No Hit
CGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCC2010470.16201024310037998No Hit
GGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAG1997510.16096588394526656No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT1939700.1563073652140082No Hit
AGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAA1927030.15528637520665578No Hit
GAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATTTTGG1913580.15420253025015304No Hit
GTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGG1854180.14941588412254975No Hit
ACGGGTTCTTCGAATGTGTGGTAGGGTGGGGGGCATCCATATAGTCACTC1849000.14899846279357692No Hit
GGCAGTGAGAGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTGAG1844080.1486019931143209No Hit
GTGGTGTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAG1825890.14713618346682758No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC1817620.14646975983929764No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA1813040.1461006884712097No Hit
GGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATTAG1793760.14454704306144217No Hit
GGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAAA1786410.14395475604060237No Hit
CTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAATGTTT1761740.14196676681555231No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC1756110.14151308301591584No Hit
GGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAA1743420.1404904813432006No Hit
GGGCAGTGAGAGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTGA1708100.1376442803124439No Hit
GTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGA1687470.13598184749068537No Hit
GCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATA1674300.1349205658492622No Hit
GTTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATG1643520.1324402128558678No Hit
GGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAA1587630.12793641399944106No Hit
GCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAG1573640.12680905407814191No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG1502500.12107636038255777No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT1483360.11953399663033006No Hit
GGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGTATGGCTA1479060.11918748857732174No Hit
GTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATAG1399520.11277789542934927No Hit
CGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCA1393290.11227586166882791No Hit
GTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTCAAGA1390810.11207601516383707No Hit
GGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCAGT1330460.10721281493149938No Hit
GGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGT1325230.10679136443911952No Hit
GGCAGGTTAGTTGTTTGTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAAT1292660.10416676739575186No Hit
GGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG1286040.10363330616065532No Hit
GGCGTTTGGTATTGGGTTATGGCAGGGGGTTTTATATTGATAATTGTTGT1276730.10288307593425824No Hit
GGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTG1273590.10263004447229403No Hit
GGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATTATT1250230.1007476193285093No Hit
GGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAAACTTCT1243980.10024397390262511No Hit
GCGGGGAAATGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGT1243670.10021899308950127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)