FastQCFastQC Report
Tue 14 May 2024
EGAF00004945338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004945338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57380940
Total Bases8.6 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGT1585140.27624852433578123No Hit
GGGGGCTAGTTTTTGTCATGTGAGAAGAAGCAGGCCGGATGTCAGAGGGG1534870.26748777555752834No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA1426380.2485808005236582No Hit
GGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGG1206810.21031548106392123No Hit
GGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGT1097020.19118194996457014No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA982580.17123804524638322No Hit
GGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT969750.1690021111539825No Hit
GGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTG873870.15229272995527782No Hit
GGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG862250.15026766727767094No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA745920.12999438489505402No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT631400.11003653826514519TruSeq Adapter, Index 18 (97% over 40bp)
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC627670.10938649663111132No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT608280.10600732577751427No Hit
GGGGCGTTTGGTATTGGGTTATGGCAGGGGGTTTTATATTGATAATTGTT589400.1027170346111444No Hit
GTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGA579830.10104923342141137No Hit
GTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATAG579390.10097255290694086No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)