FastQCFastQC Report
Sat 11 Mar 2023
EGAF00004945339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004945339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57380940
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGATCTCGCTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC883980.15405463904913375No Hit
ACAGATCTCGGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG661930.11535712032601766Illumina Single End PCR Primer 1 (97% over 41bp)
ACAGATCTCGACTAAATACTACCGTATGGCCCACCATAATTACCCCCATA586230.10216458635916385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)