FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004945340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004945340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38966179
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA2094660.5375584811638832No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT1670960.4288231596944622No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA1602150.41116425605908136No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC1438820.36924841925096125No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT1402940.36004043403896496No Hit
GGGGTAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGT1347010.34568696099250584No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG1289200.33085101826381286No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG1154740.29634417067169966No Hit
GGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGT1115540.28628416453150307No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT1109630.28476746462618263No Hit
GGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCAGT1019020.2615139657393659No Hit
GGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTG1004770.25785694820115673No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA982490.2521391692010654No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT955700.24526397622923204No Hit
GTGGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGG946730.24296197992623295No Hit
GGGGGCTAGTTTTTGTCATGTGAGAAGAAGCAGGCCGGATGTCAGAGGGG940710.2414170504118456No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC938800.2409268817453207No Hit
CTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAATGTTT908850.2332407290948389No Hit
GTGGTGTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAG893730.22936044101219163No Hit
GTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTG844260.21666481591638737No Hit
GTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATTTGGT797020.20454148198621167No Hit
GGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTAT789870.20270655739686458No Hit
GGGCAGTGAGAGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTGA767770.19703497230251907No Hit
GGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATTATT765150.19636259434110798No Hit
GTGGGGATCAATAGAGGGGGAAATAGAATGATCAGTACTGCGGCGGGTAG761340.19538482333615517No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA736340.18896900309368284No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG725710.1862409963265836No Hit
GTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATAG717800.18421103080186538No Hit
GGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCTA710890.18243769808684604No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA679540.17439225950278575No Hit
GTTGTTTTCTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATG674530.1731065291261943No Hit
GTGCTGGCAGGTCCCAGAAGCAGGAGATGGCCGAGAAGATGGTCCCGGAG661410.1697395066629448No Hit
GGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAG644410.16537674889806364No Hit
GTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGA642630.1649199424967996No Hit
GGGGCGTTTGGTATTGGGTTATGGCAGGGGGTTTTATATTGATAATTGTT638860.16395243680423477No Hit
GTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATTATTCCTT628570.16131168519243316No Hit
GGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATAT609340.15637663626192344No Hit
GCCGATGTTTCAGGTTTCTGAGTAGAGAAATGATCCGTAATATAGGCCTC606970.15576841650293707No Hit
GCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATA605570.15540913056935862No Hit
CGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGTT597080.15323031801501502No Hit
GTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAAT593220.15223971536957728No Hit
GTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTA587380.15074097976093576No Hit
GGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAACCCTGCGAAGAAAAAA585230.15018921922008313No Hit
GGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTTTAATC581380.1492011829027424No Hit
CTCAGGGTTTGTTATAATTTTTTATTTTTATGGGCTTTGGTGAGGGAGGT560960.143960740928691No Hit
GGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAACCCTGCGAAGAAAAAAA558490.14332685788873475No Hit
GGGTAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGTT552140.14169723954714677No Hit
GTGGTTTCGAAGAAGATATAAAATATGATTAGTTCTGTGGCTGTGAATGT546160.1401625753451474No Hit
GGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCG543340.1394388708217965No Hit
GGGTGAGTGAGCCTCATTGTGTTGTGGTAAATATGTAGAGGGAGTATAGG538200.1381197781799442No Hit
GGCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGG535380.13739607365659331No Hit
CCCCCCTTCGCAGTAACACCAAGTACAGGAATATTAACCTGTTTCCCATC534040.1370521856915968No Hit
GGCTGGAGTGGTAAAAGGCTCAGAAAAACCCTGCGAAGAAAAAAACTTCT532000.13652865475981105No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT525100.1347578883728887No Hit
GGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCTA516510.13255341253757522No Hit
GGCGTTTGGTATTGGGTTATGGCAGGGGGTTTTATATTGATAATTGTTGT515290.13224032050974255No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA513730.1318399733266123No Hit
GGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGTGTGAGGCGTAT511450.13125485052049882No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGG509180.13067229404248232No Hit
GTCCGGAGAGTATATTGTTGAAGAGGATAGCTATTAGAAGGATTATGGAT509090.13064919708960943No Hit
GGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAACCCTGCGAAGAAAAA504070.12936090038492098No Hit
GGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGT502320.1289117929679479No Hit
GGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAACCCTGCG499760.12825481297511876No Hit
GTGGCTGTGAATGTTATAATTAAGGAGATTTGTAGGGAGATTAGTATAGA493360.12661236299304585No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA490770.1259476840159257No Hit
CTAGGCTGGAGTGGTAAAAGGCTCAGAAAAACCCTGCGAAGAAAAAAACT487860.12520088253970194No Hit
AGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAACCCTGCGAAGAAAA473660.12155669664197764No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA470740.12080732883765688No Hit
GGGGGATAGGTGTATGAACATGAGGGTGTTTTCTCGTGTGAATGAGGGTT466080.11961141994446005No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC459330.11787914847899252No Hit
GGCAGTGAGAGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTGAG457420.11738897981246761No Hit
GTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATTA451720.11592617279718395No Hit
GGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATT451100.11576706045517061No Hit
GGGGGCTTCGACATGGGCTTTAGGGAGTCATAAGTGGAGTCCGTAAAGAG450760.11567980529987301No Hit
GGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCT447150.11475336085685998No Hit
GGGGGTTTGGATGAGAATGGCTGTTACTACGAGGGCTATGTGGCTGATTG439980.11291330361131893No Hit
GCGGTTGCTTGCGTGAGGAAATACTTGATGGCAGCTTCTGTGGAACGAGG439110.11269003306688088No Hit
GGGGGGTTCATAGTAGAAGAGCGATGGTGAGAGCTAAGGTCGGGGCGGTG433830.11133501183167074No Hit
TGTGAATGTTATAATTAAGGAGATTTGTAGGGAGATTAGTATAGAGAGGT432900.11109634331865077No Hit
GTGGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCAT430390.11045219496630654No Hit
GGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTC429550.11023662340615947No Hit
GCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTC428870.11006211309556424No Hit
GTGGCAATAAAAATGATTAAGGATACTAGTATAAGAGATCAGGTTCGTCC428610.10999538856504253No Hit
GGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGAT424920.10904841349725361No Hit
CTGGAGTGGTAAAAGGCTCAGAAAAACCCTGCGAAGAAAAAAACTTCTGA414390.10634607001112427No Hit
GGCAGGTTAGTTGTTTGTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAAT413250.10605350860806752No Hit
GGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGG412120.10576351353310778No Hit
GGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGTGTGAGGCGTA411940.10571731962736197No Hit
GCGGGGAAACGCCATATCGGGGGCACCGATTATTAGGGGAACTAGTCAGT407480.10457273729610492No Hit
GTGGGTGAGTGAGCCTCATTGTGTTGTGGTAAATATGTAGAGGGAGTATA405640.10410053292625894No Hit
GGACAGGTGGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCG405510.1040671706609981No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTAT404150.1037181500398076No Hit
GGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAG397790.10208596537012264No Hit
GGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTA397520.10201667451150394No Hit
GTTGGCTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAA393480.10097987796032042No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG392220.10065652062009979No Hit
GTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCATTG391690.10052050523095939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)