FastQCFastQC Report
Wed 8 May 2024
EGAF00004945394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004945394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72246710
Total Bases577.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCTAT7152730399.0042356254008RNA PCR Primer, Index 27 (100% over 8bp)
CTTCCTAT834370.11548899596950506No Hit
ATTACTAT746490.10332512027191273No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)