FastQCFastQC Report
Thu 12 May 2022
EGAF00004945396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004945396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72246710
Sequences flagged as poor quality0
Sequence length91
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8067171.116614168312993No Hit
TGTGGCTAGTCAGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7152670.9900340098531822No Hit
AATCCTTCCGAATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6890070.9536863339520928No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6664690.9224904497381266No Hit
AAGTTCACTCTTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6158660.8524485059596486No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6046830.8369696004150223No Hit
TGTGGCTAGTCAGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5999330.8303949065639114No Hit
TGTGGCTAGTCAGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5862600.8114694772952291No Hit
TACGCCTATAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5778640.7998481868586127No Hit
AAGTTCACTCTTTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5381480.7448754413868812No Hit
ACCTTTATGCCACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5240590.725374207351449No Hit
GCTTCCGAATTACCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5234170.7244855855719936No Hit
ACCTTTATGCCACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5077140.7027503397732575No Hit
CTACGACGTAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4493130.6219148249103661No Hit
GCTTCCGAATTACCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4170740.5772913396333204No Hit
AATCCTTCCGAATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4137430.5726807490610991No Hit
GCTTCCGAATTACCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3986700.5518175152889315No Hit
ACCTTTATGCCACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3839110.5313889033839742No Hit
AATCCTTCCGAATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3594840.49757836723637655No Hit
CTGATAGACTTGAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3567210.49375397163414086No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3508690.4856539488095721No Hit
TATTGGGACACTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3404800.4712740552476368No Hit
ACTTCCGTCGATCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3128680.43305501385461015No Hit
AGCTGTAAGTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3090460.4277648075600951No Hit
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2901210.401569843111195No Hit
AGCTGTAAGTTTCGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2641160.3655751244589546No Hit
AAGTTCACTCTTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2353460.3257532419123307No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2289480.3168974753314026No Hit
AGTATGGAGCCATATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2226970.3082451782233406No Hit
CTACGACGTAGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2215190.306614654148265No Hit
TATTGGGACACTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2213710.30640980052932515No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2209340.30580492869502296No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2084920.2885833832433338No Hit
CTGATAGACTTGAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2067620.28618881053545553No Hit
ACTTCCGTCGATCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2048600.28355616470286327No Hit
GCTCATTACCCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2011810.27846389129691856No Hit
TGAAGGCTCATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2002500.2771752513021008No Hit
GAGGTTAGTGATGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2000260.2768652025815432No Hit
GAGGTTAGTGATGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1965810.2720968193568953No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1895040.2623012175917768No Hit
ACTTCCGTCGATCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1887390.2612423458452295No Hit
CTGATAGACTTGAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1850220.2560974748884759No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1775510.24575651957023373No Hit
ATATGTCAGAGCACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1723030.23849252097431148No Hit
TATGCGAGGCTTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1563020.21634479964554787No Hit
CATTAGAGTCTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1542380.21348792214898088No Hit
GAGGTTAGTGATGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1534340.21237506870555073No Hit
GAGTCACCAATCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1489190.2061256491818105No Hit
CATTAGAGTCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1473500.20395392399183299No Hit
GCGCAACTTGATGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1463290.20254071085036263No Hit
GCGCAACTTGATGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1427800.19762837643402723No Hit
TGAAGGCTCATTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1374060.19018997543279134No Hit
GAACATTTCCGACAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1362710.18861896963889427No Hit
TTCTTTGCCTTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1352070.18714623821624543No Hit
CATTAGAGTCTGCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1347600.18652752492120403No Hit
TATTGGGACACTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1331840.1843461107087091No Hit
TGCAATTACCCGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1293810.17908220318959853No Hit
ATATGTCAGAGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1281360.1773589413275705No Hit
AGCTGTAAGTTTCGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1251580.17323695431944236No Hit
TCACCAGTACCTAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1198480.16588713866693722No Hit
GAGTCACCAATCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1188020.16443932187361887No Hit
ATATGTCAGAGCACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1178310.1630953160358444No Hit
TTCTTTGCCTTGCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1119240.15491916517721013No Hit
GAACATTTCCGACAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1111560.15385614099244102No Hit
GCTCATTACCCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1105340.152995202134464No Hit
GCGCAACTTGATGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1095370.1516152084987676No Hit
TGCAATTACCCGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1084700.150138324637897No Hit
GCTCATTACCCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1058700.14653954484570994No Hit
TGAAGGCTCATTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1047600.14500314270366083No Hit
TTCTTTGCCTTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1039210.14384184414764353No Hit
TATGCGAGGCTTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1027510.14222239324115935No Hit
TATGCGAGGCTTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020400.1412382653826036No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA968460.13404901067467295No Hit
GCGCTACTTCCTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA888790.12302151890376738No Hit
AGTATGGAGCCATATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA881430.12200278739336366No Hit
GAACATTTCCGACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA877430.12144912896379641No Hit
AGTATGGAGCCATATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA871510.1206297144880369No Hit
GGGACAGTTCGTTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA867280.12004422069876954No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG857850.11873897095106475No Hit
TGGATTCCCGGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA848750.1174793980237993No Hit
GAGATGTCTGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA847330.11728284928130292No Hit
TCCCTTGCGATTTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA825480.11425849010979186No Hit
ATTTCCATTCCCTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA823070.11392491090597759No Hit
AATCCTTCCGAATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT802130.11102650902719308No Hit
GGGACAGTTCGTTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA801940.11100021025178862No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT797230.1103482774509732No Hit
TTACCTGGTATCCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA795530.11011297261840712No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA784510.10858764364494937No Hit
TGCAATTACCCGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA769480.10650727209585045No Hit
GTTAGTGTTAGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA767220.10619445508314497No Hit
GGATAACCGCGCTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA743050.10284897402248488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)