FastQCFastQC Report
Thu 2 May 2024
EGAF00004945405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004945405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48996075
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC27

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5181151.0574622559051923No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3380930.6900409879770981No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3171900.6473783869422194No Hit
AATCCTTCCGAATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2929070.5978172741387958No Hit
TACGCCTATAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2462120.5025137217624065No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2168210.442527284073265No Hit
CTACGACGTAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140320.43683499137430903No Hit
ATATGTCAGAGCACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1882200.384153220436535No Hit
ACCTTTATGCCACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1852090.37800783021905326No Hit
ACCTTTATGCCACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1821550.3717746778696049No Hit
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1792520.3658497134719465No Hit
GCGCAACTTGATGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1789470.36522721462892693No Hit
GCGCAACTTGATGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1713840.3497912843018548No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1647670.33628612087804993No Hit
ATATGTCAGAGCACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1623630.3313796054071678No Hit
AATCCTTCCGAATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1606160.32781401367354424No Hit
AATCCTTCCGAATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1589390.32439129052684323No Hit
TATTGGGACACTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1508390.307859354040094No Hit
ACCTTTATGCCACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1479980.30206093039085274No Hit
TGTGGCTAGTCAGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1476630.30137720215343783No Hit
TGTGGCTAGTCAGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1339490.2733872049955022No Hit
GCGCAACTTGATGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1323540.2701318421934818No Hit
ATATGTCAGAGCACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1252660.2556653772776697No Hit
CTACGACGTAGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1159890.23673120755080893No Hit
TGTGGCTAGTCAGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140880.23285130492595582No Hit
TATTGGGACACTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1105480.22562623638730245No Hit
GAGGTTAGTGATGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1003300.2047715046562403No Hit
CTGATAGACTTGAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1001130.2043286120367805No Hit
GAGGTTAGTGATGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA986010.201242650559254No Hit
CTGTAGCCGCCTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA942070.19227458526014585No Hit
CTGTAGCCGCCTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA902700.18423924773566044No Hit
GTATCCTTCCTTGGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA896300.18293302065522596No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA858100.1751364777688825No Hit
GAACATTTCCGACAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA853410.1741792582365016No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA838300.17109533773878827No Hit
GCTTCCGAATTACCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA801790.16364372044087205No Hit
GAGGTTAGTGATGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA776680.1585188201299798No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA776600.15850249229147437No Hit
AAGTTCACTCTTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA768590.15686766746111808No Hit
GTATCCTTCCTTGGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA704730.14383397037415752No Hit
GCTTCCGAATTACCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA687870.14039287840913786No Hit
AAGTATGCCCTACGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA687010.1402173541452045No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA662160.1351455193094549No Hit
CTTCCGATTCATTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA661770.1350659210967409No Hit
AAGTTCACTCTTTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA658410.1343801518795128No Hit
GAACATTTCCGACAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA658370.13437198796026006No Hit
CATTAACGGGATGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA634760.12955323462134466No Hit
GCTTCCGAATTACCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA603590.12319150054366598No Hit
CATTAACGGGATGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA595840.12160974118845234No Hit
GATTAACCAGCTCATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA595270.12149340533910115No Hit
GATTAACCAGCTCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA588380.12008717024782087No Hit
ATTTCCATTCCCTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA582480.11888299215804532No Hit
TATTGGGACACTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA578790.1181298706069823No Hit
TATGCGAGGCTTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA574000.1171522412764696No Hit
GTATCCTTCCTTGGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA561730.11464795904569908No Hit
CTGATAGACTTGAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA559370.11416628780978885No Hit
AAGTATGCCCTACGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA559060.1141030174355803No Hit
GAACATTTCCGACAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA552920.11284985583028845No Hit
AAGTATGCCCTACGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA552630.11279066741570626No Hit
TTCCGAGGATGATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA542400.11070274506682423No Hit
GAGTCACCAATCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA534180.10902505966039118No Hit
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT504320.10293069393823893No Hit
TTCCGAGGATGATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA491920.10039987896989708No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)