FastQCFastQC Report
Wed 8 May 2024
EGAF00004945408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004945408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48996075
Total Bases391.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACTAAT4854055899.07029899844018RNA PCR Primer, Index 29 (100% over 8bp)
CAACTAAA708900.14468505895625314No Hit
AAACTAAT665270.13578026403135351No Hit
CAACAAAT612450.12499980865814252No Hit
NAACTAAT568860.11610317765249564No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)