FastQCFastQC Report
Wed 8 May 2024
EGAF00004945410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004945410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74367369
Total Bases594.9 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC24

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAGAT7362276998.99875441337718RNA PCR Primer, Index 28 (100% over 8bp)
CAAAAAAT1821910.24498782523824394No Hit
CAAAATAT961490.1292892316790177No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)