FastQCFastQC Report
Mon 13 May 2024
EGAF00004945420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004945420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375653991
Total Bases34.3 Gbp
Sequences flagged as poor quality0
Sequence length91-98
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT44523701.1852316511126857Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7376970.19637672370689654Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5304650.14121106462569166No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)