FastQCFastQC Report
Wed 15 May 2024
EGAF00004945426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004945426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74367369
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATCCTTGTGAGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7139500.960031273931447No Hit
AATCCTTCCGAATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6252980.8408230765834945No Hit
AATCCTTCCGAATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3713470.4993413172919967No Hit
CTACGACGTAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3587740.4824347086959605No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3536530.47554862402083903No Hit
AATCCTTCCGAATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3255630.4377766813291458No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3214670.4322688893296736No Hit
GCTTCCGAATTACCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3190080.42896233158389674No Hit
TATATCCTTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3124560.42015201586599094No Hit
TACGCCTATAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2846960.3828238161820677No Hit
TGTGGCTAGTCAGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2846240.382726999525827No Hit
GCTTCCGAATTACCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2607120.35057311224765797No Hit
CTTCCGATTCATTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2571120.3457322794356218No Hit
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2517530.33852616192459356No Hit
GCTTCCGAATTACCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2496160.3356525897803377No Hit
CTGATAGACTTGAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2490350.3348713331515063No Hit
ACCTTTATGCCACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2461840.33103766249952987No Hit
TGTGGCTAGTCAGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2369810.3186626112858719No Hit
TGTGGCTAGTCAGTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2281470.3067837454354476No Hit
GCGCAACTTGATGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2212120.29745841889337243No Hit
ACCTTTATGCCACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2187430.29413841438978433No Hit
GCGCAACTTGATGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2123180.2854988724960809No Hit
ACCTTTATGCCACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1805960.24284306736735572No Hit
CTACGACGTAGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1793230.24113129509798847No Hit
TATTGGGACACTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750000.2353182616962017No Hit
TATATCCTTGTGAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1737400.23362397021198908No Hit
GAGGTTAGTGATGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1670150.22458102558394932No Hit
GAGGTTAGTGATGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1669880.22454471933785908No Hit
GCGCAACTTGATGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1637720.22022024202577342No Hit
TATGCGAGGCTTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1604980.21581777351838277No Hit
CAGCCCACTGCAATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1574540.21172458044065No Hit
CTTCCGATTCATTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1457160.1959407761218499No Hit
CTGATAGACTTGAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1397070.1878606193530929No Hit
AGCTGTAAGTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1346700.18108748744358566No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1325040.17817491970167723No Hit
GAGGTTAGTGATGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1281670.1723430608389548No Hit
CTTCCGATTCATTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1269740.17073886263207727No Hit
GAGGCACTTAACATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1264380.17001811641339631No Hit
CTGATAGACTTGAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1231830.16564119674584696No Hit
GAGATGTCTGCAACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1195570.16076540236350165No Hit
TATTGGGACACTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1189030.15988598440264842No Hit
ACTTCCGTCGATCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1163000.15638579334439007No Hit
AGCTGTAAGTTTCGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140230.15332396659077718No Hit
AAGTTCACTCTTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1133780.15245665071195405No Hit
GCTGCGCTTTCCATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1127010.15154630520813503No Hit
CCTTCCCTTTCCTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1126510.151479071419079No Hit
CTGTAGCCGCCTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1099760.1478820637045799No Hit
CTGTAGCCGCCTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1096750.14747731629446242No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1088010.14630206966176254No Hit
TATGCGAGGCTTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1079220.14512009965015704No Hit
TATGCGAGGCTTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1067380.1435280035253096No Hit
TGGATTCCCGGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1062330.14284894225584344No Hit
GAGGCACTTAACATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1035230.1392048708890051No Hit
CTTACCTAGTCATTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1007150.1354290212956169No Hit
GGACGCTTTCCTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1002500.13480374705739556No Hit
AAGTTCACTCTTTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA994180.13368497680750277No Hit
GAGATGTCTGCAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA989980.13312021297943188No Hit
GGACGCTTTCCTTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA976620.13132372613585402No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA969430.1303569042492279No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA943190.126828474999566No Hit
TCACCAGTACCTAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA938900.12625160908946503No Hit
TTGTTGTATCCGATCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA922150.1239992771560871No Hit
ATATGTCAGAGCACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA921940.12397103896468356No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA907090.12197419542971866No Hit
CCAAAGTAAGAGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA905910.12181552368754635No Hit
GCTCATTACCCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA897430.12067523862515561No Hit
CCTACACCTCATAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA893250.12011316414864696No Hit
GAGGCACTTAACATACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA892850.12005937711740211No Hit
GACTGGGAGGGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890990.11980926742211359No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA879160.11821851597304726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG866790.1165551520318004No Hit
GGACGCTTTCCTTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA853050.11470756750853993No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA822080.11054310661440772No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA815380.10964217384105655No Hit
ACTTCCGTCGATCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA813640.10940820025514147No Hit
CGCCACTTCATTCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810170.10894159775909243No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA801480.10777307450529816No Hit
CAATCAGACCTATGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA797140.10718948521629157No Hit
GCTGCGCTTTCCATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA793400.10668657647415226No Hit
GCTAGTTTGTGCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA787660.1059147325757887No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA787100.10583943073204592No Hit
GTATCCTTCCTTGGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA778060.10462384382591242No Hit
CCTTCCCTTTCCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA772600.10388965084942026No Hit
GCTCCTTTACACGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA763670.10268885537687907No Hit
GAGTCACCAATCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760090.1022074614472377No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)